Zhang Doudou, Zhao Yu, Lan Dandan, Pang Xiaomin, Bai Ligai, Liu Haiyan, Yan Hongyuan
College of Pharmaceutical Sciences, Hebei University, Baoding, 071002, China; Key Laboratory of Medicinal Chemistry and Molecular Diagnosis, Ministry of Education, Hebei University, Baoding, 071002, China.
North China Pharmaceutical Corporation New Drug Research & Development Company Ltd., Shijiazhuang, 050015, China.
J Chromatogr B Analyt Technol Biomed Life Sci. 2017 Nov 15;1068-1069:358-364. doi: 10.1016/j.jchromb.2017.11.003. Epub 2017 Nov 4.
In this work a polymer monolithic column was fabricated within the confines of a stainless steel column (50×4.6mm i.d.) via radical polymerization by using iron porphyrin and butyl methacrylate as co-monomers, ethylene glycol dimethacrylate as crosslinking agent, ethylene glycol, isopropyl alcohol and N, N-dimethylformamide as tri-porogens, benzoyl peroxide and N,N-dimethylaniline as initiators. The resulting monolithic column was characterized by elemental analysis, scanning electron microscopy, nitrogen adsorption BET surface area, and mercury intrusion porosimetry, respectively. Results showed that the homemade monolith occupied relatively uniform pore structure, low back pressure, and enhanced selectivity for proteins in complex bio-samples. The present work described a simple and efficient method for "fractionation separation" of human plasma proteins, and it is a promising separation method for complex bio-samples in proteomic research.
在本工作中，通过自由基聚合，以铁卟啉和甲基丙烯酸丁酯作为共聚单体，乙二醇二甲基丙烯酸酯作为交联剂，乙二醇、异丙醇和N，N -二甲基甲酰胺作为三元致孔剂，过氧化苯甲酰和N，N -二甲基苯胺作为引发剂，在不锈钢柱（内径50×4.6mm）内制备了聚合物整体柱。分别采用元素分析、扫描电子显微镜、氮吸附BET表面积和压汞法对所得整体柱进行了表征。结果表明，自制的整体柱具有相对均匀的孔结构、较低的背压以及对复杂生物样品中蛋白质增强的选择性。本工作描述了一种用于人血浆蛋白“分级分离”的简单高效方法，并且是蛋白质组学研究中用于复杂生物样品的一种有前景的分离方法。
