Seif M W, Aplin J D, Buckley C H
Department of Obstetrics and Gynaecology, University of Manchester at St. Mary's Hospital, Manchester, United Kingdom.
Fertil Steril. 1989 Feb;51(2):273-9. doi: 10.1016/s0015-0282(16)60490-9.
Monoclonal antibody D9B1 has been shown to bind to a carbohydrate epitope associated with high molecular weight secretory sialoglycoprotein(s) of human endometrium. The authors demonstrate that, in a group of 28 patients diagnosed on the basis of histopathologic assessment as exhibiting luteal phase defect, 68% reveal significantly diminished expression of the epitope. Furthermore, histologic assessment of the secretory activity in single glands does not correlate simply with the level of expression of the epitope. Of specimens from patients complaining of infertility, 92% showed defective production of the D9B1 epitope. Thus, D9B1 immunohistochemistry provides a new molecular criterion for defective endometrial function.
单克隆抗体D9B1已被证明可与人子宫内膜高分子量分泌性唾液糖蛋白相关的碳水化合物表位结合。作者证明，在一组根据组织病理学评估诊断为黄体期缺陷的28例患者中，68%的患者该表位表达明显减少。此外，单个腺体分泌活性的组织学评估与该表位的表达水平并非简单相关。在主诉不孕的患者标本中，92%显示D9B1表位产生缺陷。因此，D9B1免疫组织化学为子宫内膜功能缺陷提供了一个新的分子标准。
