Liu Juan, Peng Cheng, Zhou Qin-Mei, Guo Li, Liu Zhao-Hua, Xiong Liang
State Key Laboratory Breeding Base of Systematic Research, Development and Utilization of Chinese Medicine Resources, Chengdu University of Traditional Chinese Medicine, Chengdu, 611137, China; School of Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu, 611137, China.
State Key Laboratory Breeding Base of Systematic Research, Development and Utilization of Chinese Medicine Resources, Chengdu University of Traditional Chinese Medicine, Chengdu, 611137, China; School of Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu, 611137, China.
Phytochemistry. 2018 Jan;145:128-136. doi: 10.1016/j.phytochem.2017.11.003. Epub 2017 Nov 8.
The crude extract and some Chinese patented medicines of Leonurus japonicus Houtt. have been proven to affect the uterine smooth muscle. L. japonicus injection is widely used in obstetric departments in China for treating postpartum hemorrhage caused by uterine inertia. Bioassay-guided isolation of the 95% EtOH extract of L. japonicus yielded four cyclopeptides, nine alkaloids, and three flavonoid glycosides, including two previously undescribed cyclopeptides, namely, cycloleonuripeptide G and cycloleonuripeptide H. The structures of the cyclopeptides were elucidated to be cyclo-(L-Phe-L-Phe-Gly-L-Pro-Gly-L-Pro) and cyclo-(L-Phe-L-Ala-L-Pro-L-Ile-L-His-Gly-L-Ala-L-Pro), respectively, via spectroscopic and chemical methods. Cyclopeptides (cycloleonuripeptides C and D) and alkaloids (imperialine-3β-D-glucoside and leonurine) promoted contraction of uterine smooth muscle strips isolated from normal rats. However, it was observed that flavonoid glycosides (spinosin, linarin, and apigenin-7-O-β-D-glucopyranoside) significantly inhibited contraction of the uterine smooth muscle strips.
益母草的粗提取物和一些中成药已被证明会影响子宫平滑肌。益母草注射液在中国妇产科被广泛用于治疗子宫收缩乏力引起的产后出血。通过生物活性追踪法对益母草95%乙醇提取物进行分离，得到了4种环肽、9种生物碱和3种黄酮苷，其中包括2种此前未被描述的环肽，即环益母草肽G和环益母草肽H。通过光谱和化学方法确定，这两种环肽的结构分别为环-（L-苯丙氨酸-L-苯丙氨酸-甘氨酸-L-脯氨酸-甘氨酸-L-脯氨酸）和环-（L-苯丙氨酸-L-丙氨酸-L-脯氨酸-L-异亮氨酸-L-组氨酸-甘氨酸-L-丙氨酸-L-脯氨酸）。环肽（环益母草肽C和D）和生物碱（异欧前胡素-3β-D-葡萄糖苷和益母草碱）可促进从正常大鼠分离的子宫平滑肌条收缩。然而，研究发现黄酮苷（刺槐素、蒙花苷和芹菜素-7-O-β-D-吡喃葡萄糖苷）可显著抑制子宫平滑肌条的收缩。
