Center for Gastrointestinal Biology, Departamento de Morfologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil.
Center for Gastrointestinal Biology, Departamento de Morfologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil.
Int J Biochem Cell Biol. 2018 Jan;94:1-5. doi: 10.1016/j.biocel.2017.11.004. Epub 2017 Nov 8.
Intravital imaging has been increasingly employed in cell biology studies and it is becoming one of the most powerful tools for in vivo investigation. Although some protocols can be extremely complex, most intravital imaging procedures can be performed using basic surgery and animal maintenance techniques. More importantly, regular confocal microscopes - the same that are used for imaging immunofluorescence slides - can also acquire high quality intravital images and movies after minor adaptations. Here we propose minimal adaptations in stock microscopes that allow major improvements in different fields of scientific investigation.
活体成像技术在细胞生物学研究中得到了越来越广泛的应用，它已成为活体研究中最强大的工具之一。尽管一些方案可能非常复杂，但大多数活体成像程序都可以使用基本的手术和动物维持技术来进行。更重要的是，经过一些简单的改造，常规的共聚焦显微镜（也就是用于免疫荧光载玻片成像的显微镜）也可以获取高质量的活体成像图片和视频。在此，我们提出了对库存显微镜的最小改造方案，这些方案可以在不同的科学研究领域带来重大的改进。
