基于 HKUST-1 和 QDs-鲁米诺-适配体缀合物的选择性和灵敏检测腺苷的 turn-on 化学发光生物传感器。
A turn-on chemiluminescence biosensor for selective and sensitive detection of adenosine based on HKUST-1 and QDs-luminol-aptamer conjugates.
机构信息
Key Laboratory of Interfacial Reaction & Sensing Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, PR China.
Key Laboratory of Interfacial Reaction & Sensing Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, PR China.
出版信息
Talanta. 2018 May 15;182:116-124. doi: 10.1016/j.talanta.2018.01.065. Epub 2018 Jan 31.
In this work, HKUST-1 and QDs-luminol-aptamer conjugates were prepared. The QDs-luminol-aptamer conjugates can be adsorbed by graphene oxide through π-π conjugation. When the adenosine was added, the QDs-luminol-aptamer conjugates were released from magnetic graphene oxide (MGO), the chemiluminescent switch was turned on. It was reported that HKUST-1 can catalyze the chemiluminescence reaction of luminol-HO system in an alkaline medium, and improve the chemiluminescence resonance energy transfer (CRET) between chemiluminescence and QDs indirectly. Thus, the adenosine can be detected sensitively. Based on this phenomenon, the excellent platform for detection of adenosine was established. Under the optimized conditions, the linear detection range for adenosine was 1.0 × 10-2.2 × 10 mol/L with a detection limit of 2.1 × 10 mol/L. The proposed method was successfully used for adenosine detection in biological samples.
在这项工作中，制备了 HKUST-1 和 QDs-鲁米诺-适体缀合物。QDs-鲁米诺-适体缀合物可以通过π-π 共轭被氧化石墨烯吸附。当加入腺嘌呤时，QDs-鲁米诺-适体缀合物从磁性氧化石墨烯（MGO）上释放出来，化学发光开关被打开。据报道，HKUST-1 可以在碱性介质中催化鲁米诺-HO 体系的化学发光反应，间接提高化学发光与 QDs 之间的化学发光共振能量转移（CRET）。因此，可以灵敏地检测腺苷。基于这一现象，建立了检测腺苷的优良平台。在优化条件下，腺苷的线性检测范围为 1.0×10-2.2×10 mol/L，检测限为 2.1×10 mol/L。该方法成功用于生物样品中腺苷的检测。
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