School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, China.
School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, China.
Sci Total Environ. 2018 Mar;616-617:531-542. doi: 10.1016/j.scitotenv.2017.10.275. Epub 2017 Nov 10.
The environmental and human health risks of veterinary drugs are becoming public health issues. Enrofloxacin (EF) is an extensively used animal-specific antibacterial agent that leaves drug residues in the environment. This study investigated the proteomic response of the earthworm Eisenia fetida to EF exposure. Earthworms were exposed to EF in soil at 1-500mg·kg, and samples were collected at intervals during a 28 day period. The extracted proteins were separated by two dimensional electrophoresis to detect differentially expressed proteins (DEPs) in EF-exposed earthworms. In total, 35 unique DEPs were found. These proteins were subjected to MALDI-TOF/TOF-MS analysis and identified through comparison of their mass spectra with those in protein databases. The DEPs were grouped on the basis of their function, into metabolism, stress-related, transport, transcription, and predicted/hypothetical protein categories. Knowledge of proteins that are induced or repressed by EF in earthworms could provide insight into mechanisms of sub-clinical physiological effects of xenobiotic residues in the environment, and may also help understand synergy between pollutants. As several DEPs in E. fetida showed similarity to human protein sequences, E. fetida has potential as an indicator species to assess the environmental and biological risks of drug residues.
兽药对环境和人类健康的风险正成为公共卫生问题。恩诺沙星（EF）是一种广泛应用于动物的特定抗菌药物，会在环境中留下药物残留。本研究调查了蚯蚓对 EF 暴露的蛋白质组学反应。将蚯蚓暴露于土壤中的 EF 中，浓度为 1-500mg·kg，并在 28 天的时间间隔内采集样本。提取的蛋白质通过二维电泳分离，以检测 EF 暴露的蚯蚓中差异表达的蛋白质（DEPs）。总共发现了 35 个独特的 DEPs。通过将它们的质谱与蛋白质数据库中的质谱进行比较，对这些蛋白质进行 MALDI-TOF/TOF-MS 分析和鉴定。根据它们的功能，将 DEPs 分为代谢、应激相关、运输、转录和预测/假设蛋白类别。了解 EF 在蚯蚓中诱导或抑制的蛋白质可以深入了解环境中外源生物残留的亚临床生理效应的机制，也有助于理解污染物之间的协同作用。由于几种 DEPs 在 E. fetida 中与人类蛋白质序列相似，因此 E. fetida 有可能成为评估药物残留的环境和生物风险的指示物种。
