Widders P R, Dorrance L A, Yarnall M, Corbeil L B
Department of Veterinary Microbiology and Pathology, Washington State University, Pullman 99164-7040.
Infect Immun. 1989 Feb;57(2):639-42. doi: 10.1128/iai.57.2.639-642.1989.
Nonimmune binding of immunoglobulin to whole bacteria was quantitated for North American isolates of Haemophilus somnus recovered from cattle with pneumonia, reproductive failure (abortion), or thromboembolic meningoencephalitis or from the vagina or prepuce of carrier cattle. Quantitative binding activity covered a wide range, with most pathogenic and carrier isolates demonstrating significant immunoglobulin-Fc binding. Isolates for which Fc binding was not detectable were recovered only from the prepuces of asymptomatic bulls. Expression of Fc-binding activity correlated with the presence of the 41,000-molecular-weight protein (41K protein) and 270K protein. Isolates that lacked Fc-binding activity did not possess 41K or 270K protein. A 33K protein was detected in isolates that lacked Fc-binding activity but not in isolates that bound Fc.
对从患有肺炎、生殖机能衰竭（流产）或血栓栓塞性脑膜脑炎的牛或带菌牛的阴道或包皮中分离出的北美睡眠嗜血杆菌菌株，测定了免疫球蛋白与全菌的非免疫结合情况。定量结合活性范围很广，大多数致病菌株和带菌菌株都显示出显著的免疫球蛋白-Fc结合。仅从无症状公牛的包皮中分离出未检测到Fc结合的菌株。Fc结合活性的表达与41,000分子量蛋白（41K蛋白）和270K蛋白的存在相关。缺乏Fc结合活性的菌株不具有41K或270K蛋白。在缺乏Fc结合活性的菌株中检测到一种33K蛋白，但在结合Fc的菌株中未检测到。
