Stephens L R, Little P B, Wilkie B N, Barnum D A
Am J Vet Res. 1981 Mar;42(3):468-73.
Of 23 cattle inoculated IV with Haemophilus somnus, 16 (70%) died of thromboembolic meningoencephalitis. The inoculum was prepared from a minimally subcultured isolate of H somnus that was passaged through a calf by intracisternal inoculation immediately before use. Serum antibody titers, as measured by 7 serologic tests, were not correlated with the animal's susceptibility to infection. All cattle that died had a mean 4-fold increase in agglutination titer during the acute phase of the disease, 2 to 4 days after inoculation. Similarly, high acute-phase titers were demonstrated in 15 cattle with naturally occurring disease. Haemophilus somnus was isolated more frequently and in greater numbers from the CNS and urinary tract than from other organs of cattle that died.
23头经静脉接种睡眠嗜血杆菌的牛中，16头（70%）死于血栓栓塞性脑膜脑炎。接种物由一株传代次数极少的睡眠嗜血杆菌分离株制备而成，该分离株在使用前通过脑池内接种在一头小牛体内传代。通过7种血清学检测方法测得的血清抗体滴度与动物对感染的易感性无关。所有死亡的牛在接种后2至4天疾病急性期的凝集滴度平均升高4倍。同样，15头自然发病的牛也表现出急性期高滴度。与死亡牛的其他器官相比，从其中枢神经系统和泌尿道分离出睡眠嗜血杆菌的频率更高、数量更多。
