在牛分枝杆菌感染部位测量牛γδ T细胞功能。
Measuring bovine γδ T cell function at the site of Mycobacterium bovis infection.
作者信息
Rusk Rachel A, Palmer Mitchell V, Waters W Ray, McGill Jodi L
机构信息
Pathobiology Graduate Program, Department of Diagnostic Medicine/Pathobiology, College of Veterinary Medicine, Kansas State University, Manhattan, KS, 66506, USA.
Infectious Bacterial Diseases Research Unit, National Animal Disease Center, Agricultural Research Service, USDA, Ames, IA, USA.
出版信息
Vet Immunol Immunopathol. 2017 Dec;193-194:38-49. doi: 10.1016/j.vetimm.2017.10.004. Epub 2017 Oct 27.
Abstract
Bovine γδ T cells are amongst the first cells to accumulate at the site of Mycobacterium bovis infection; however, their role in the developing lesion remains unclear. We utilized transcriptomics analysis, in situ hybridization, and a macrophage/γδ T cell co-culture system to elucidate the role of γδ T cells in local immunity to M. bovis infection. Transcriptomics analysis revealed that γδ T cells upregulated expression of several novel, immune-associated genes in response to stimulation with M. bovis antigen. BCG-infected macrophage/γδ T cell co-cultures confirmed the results of our RNAseq analysis, and revealed that γδ T cells from M. bovis-infected animals had a significant impact on bacterial viability. Analysis of γδ T cells within late-stage M. bovis granulomas revealed significant expression of IFN-γ and CCL2, but not IL-10, IL-22, or IL-17. Our results suggest γδ T cells influence local immunity to M. bovis through cytokine secretion and direct effects on bacterial burden.
摘要
牛γδ T细胞是最早在牛分枝杆菌感染部位聚集的细胞之一；然而，它们在病变发展过程中的作用仍不清楚。我们利用转录组学分析、原位杂交和巨噬细胞/γδ T细胞共培养系统来阐明γδ T细胞在牛分枝杆菌感染局部免疫中的作用。转录组学分析表明，γδ T细胞在受到牛分枝杆菌抗原刺激后上调了几个新的免疫相关基因的表达。卡介苗感染的巨噬细胞/γδ T细胞共培养证实了我们RNA测序分析的结果，并表明来自牛分枝杆菌感染动物的γδ T细胞对细菌活力有显著影响。对晚期牛分枝杆菌肉芽肿内的γδ T细胞分析显示，IFN-γ和CCL2有显著表达，但IL-10、IL-22或IL-17没有。我们的结果表明，γδ T细胞通过细胞因子分泌和对细菌负荷的直接影响来影响对牛分枝杆菌的局部免疫。
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