青海湖裸鲤精子冷冻保存条件的研究。
Studies of the cryopreservation condition of Gymnocypris przewalskii spermatozoa.
作者信息
Wei Fulei, Yu Luxian, Li Ruihong, Zhang Xia, Zhang Xuehan, Zhang Ya, Wang Yuqing, Wang Hongchao, Liang Jian, Ma Rui, Qi Hongfang, Qin Qiwei, Zhang Rongqing, Zhu Shihai, Li Changzhong
机构信息
State Key Laboratory of Plateau Ecology and Agriculture, Qinghai University, Xining, 810016, China.
Gymnocypris przewalskii Rescue Centre, Xining, 810016, China.
出版信息
Anim Reprod Sci. 2018 Jan;188:13-20. doi: 10.1016/j.anireprosci.2017.10.021. Epub 2017 Nov 7.
The endemic naked carp (Gymnocypris przewalskii Kessler, 1876) plays an important role in the maintenance of the distinctive ecological system of Lake Qinghai at 3.2km altitude on the Qinghai-Tibet Plateau in China. This study aimed to develop a cryopreservation protocol for Gymnocypris przewalskii spermatozoa. Semen was collected from mature individuals during migration and frozen using the liquid nitrogen vapor method. The influence of different cryoprotectants and three extenders on the post-thaw quality of the sperm was analyzed. The highest sperm motility rate and longest motility time after cryopreservation were achieved by combining Ringer's solution with 15% ethylene glycol (P<0.05). The fertilization rate of this cryopreserved semen was 15.26±4.54%. This study thus provides a valuable method for the cryopreservation of the sperm of this important endangered fish species.
青海裸鲤（Gymnocypris przewalskii Kessler，1876）在中国青藏高原海拔3.2千米的青海湖独特生态系统的维持中发挥着重要作用。本研究旨在开发一种青海裸鲤精子的冷冻保存方案。在洄游期间从成熟个体采集精液，并使用液氮蒸汽法进行冷冻。分析了不同冷冻保护剂和三种稀释液对解冻后精子质量的影响。将林格氏液与15%乙二醇混合时，冷冻保存后的精子活力率最高且活力时间最长（P<0.05）。这种冷冻精液的受精率为15.26±4.54%。因此，本研究为这种重要濒危鱼类的精子冷冻保存提供了一种有价值的方法。
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