Nigovic Biljana, Milanovic Ivona
University of Zagreb, Faculty of Pharmacy and Biochemistry.
Anal Sci. 2017;33(11):1219-1223. doi: 10.2116/analsci.33.1219.
The electrooxidation of xanthine oxidase inhibitor febuxostat was investigated on a boron-doped diamond electrode in aqueous solution. The oxidation of the drug molecule was irreversible and exhibited a diffusion-controlled form. A green electroanalytical method for simple and fast measurements of febuxostat was developed at the unmodified electrode surface. The analyses were performed using square-wave voltammetric peak current at 1.38 V. The linear response was obtained in the range of 7.5 × 10 - 2.0 × 10 M with the detection limit of 9.5 × 10 M. The practical analytical value of the method is demonstrated by quantitative determination of febuxostat in film-coated tablets with excellent recovery of 99.6%. Interference studies reveal that uric acid shows a well-developed voltammetric response at +0.64 V. In view of this, the electroanalytical performances of the boron-doped diamond electrode can open up new possibilities for development of the method for simultaneous clinical analysis of febuxostat and uric acid.
在水溶液中，研究了黄嘌呤氧化酶抑制剂非布索坦在硼掺杂金刚石电极上的电氧化过程。药物分子的氧化是不可逆的，呈现出扩散控制的形式。在未修饰的电极表面开发了一种用于简单快速测定非布索坦的绿色电分析方法。分析采用1.38 V处的方波伏安峰电流进行。在7.5×10⁻².0×10 M范围内获得线性响应，检测限为9.5×10 M。通过对薄膜包衣片中的非布索坦进行定量测定，回收率高达99.6%，证明了该方法的实际分析价值。干扰研究表明，尿酸在+0.64 V处呈现出良好的伏安响应。鉴于此，硼掺杂金刚石电极的电分析性能为开发同时临床分析非布索坦和尿酸的方法开辟了新的可能性。
