Du Huanan, Zhang Xu, Zhang Ruchun, Zhang Lu, Yu Dianyu, Jiang Lianzhou
School of Food Science, Northeast Agricultural University.
J Oleo Sci. 2017 Dec 1;66(12):1301-1310. doi: 10.5650/jos.ess17006. Epub 2017 Nov 13.
Rosa acicularis seed oil was extracted from Rosa acicularis seeds by the ultrasonic-assisted aqueous enzymatic method using cellulase and protease. Based on a single experiment, Plackett-Burman design was applied to ultrasonic-assisted aqueous enzymatic extraction of wild rose seed oil. The effects of enzyme amount, hydrolysis temperature and initial pH on total extraction rate of wild rose seed oil was studied by using Box-Behnken optimize methodology. Chemical characteristics of a sample of Rosa acicularis seeds and Rosa acicularis seed oil were characterized in this work. The tocopherol content was 200.6±0.3 mg/100 g oil. The Rosa acicularis seed oil was rich in linoleic acid (56.5%) and oleic acid (34.2%). The saturated fatty acids included palmitic acid (4%) and stearic acid (2.9%). The major fatty acids in the sn-2 position of triacylglycerol in Rosa acicularis oil were linoleic acid (60.6%), oleic acid (33.6%) and linolenic acid (3.2%). According to the 1,3-random-2-random hypothesis, the dominant triacylglycerols were LLL (18%), LLnL (1%), LLP (2%), LOL (10%), LLSt (1.2%), PLP (0.2%), LLnP (0.1%), LLnO (0.6%) and LOP (1.1%). This work could be useful for developing applications for Rosa acicularis seed oil.
刺果蔷薇籽油采用超声波辅助水酶法，利用纤维素酶和蛋白酶从刺果蔷薇种子中提取。基于单因素实验，采用Plackett-Burman设计法对超声波辅助水酶法提取野玫瑰籽油进行研究。利用Box-Behnken优化方法研究了酶用量、水解温度和初始pH对野玫瑰籽油总提取率的影响。本研究对刺果蔷薇种子样品和刺果蔷薇籽油的化学特性进行了表征。生育酚含量为200.6±0.3mg/100g油。刺果蔷薇籽油富含亚油酸（56.5%）和油酸（34.2%）。饱和脂肪酸包括棕榈酸（4%）和硬脂酸（2.9%）。刺果蔷薇油中甘油三酯sn-2位的主要脂肪酸为亚油酸（60.6%）、油酸（33.6%）和亚麻酸（3.2%）。根据1,3-随机-2-随机假说，主要的甘油三酯为LLL（18%）、LLnL（1%）、LLP（2%）、LOL（10%）、LLSt（1.2%）、PLP（0.2%）、LLnP（0.1%）、LLnO（0.6%）和LOP（1.1%）。这项工作可能有助于开发刺果蔷薇籽油的应用。
