Laus Ana Carolina, da Silva Ismael Dale Cotrim Guerreiro, Cordeiro Fernanda Bertuccez, Lo Turco Edson Guimarães, de Souza Viana Luciano, Carvalho André Lopes
Molecular Oncology Research Center, Barretos Cancer Hospital, Pio XII, 1331, Antenor Duarte Villela St, Barretos, SP, 14784-400, Brazil.
Surgery Department, Urology Discipline, Universidade Federal de Sao Paulo Sao Paulo, Sao Paulo, Brazil.
Pathol Oncol Res. 2018 Oct;24(4):931-935. doi: 10.1007/s12253-017-0336-x. Epub 2017 Nov 13.
In the last decade organ preservation protocols based on chemoradiotherapy (CRT) has been showing the possibility of preserving function without jeopardizing survival for locally advanced head and neck squamous cell carcinoma (HNSCC). Still, only a percentage of the patients will benefit from this approach and, to date, no biomarkers are known to correctly predict these patients. More recently, modern mass spectrometry method has been used to determine metabolic profiles, and lipidomics, in particular, emerged as a new field of study in oncology and other diseases. This study aimed to analyze the lipid profile on saliva from patients undergoing to a prospective, single center, open-label, non-randomized phase II trial for organ preservation on HNSCC. The lipid analysis was performed using matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF-MS). Multivariate statistical analyses based on principal component analysis and orthogonal partial least square-discriminant analysis were applied to MALDI-TOF-MS data to visualize differences between the lipid profiles and identify potential biomarkers. The results assisted on distinguishing complete responders from non-responders to the treatment protocol. In conclusion, we demonstrated that a group of lipids is differentially abundant in saliva from HNSCC patients submitted to an organ preservation protocol, being able to differentiate responders from non-responders. These results suggest the potential use of lipid biomarkers to identify patients who may benefit from this treatment. Also, we showed that saliva testing might be routinely used in clinical practice, for being a non-invasive alternative to blood testing, besides inexpensive and easy to obtain.
在过去十年中，基于放化疗（CRT）的器官保存方案已显示出对于局部晚期头颈部鳞状细胞癌（HNSCC）在不危及生存的情况下保留功能的可能性。然而，只有一部分患者能从这种方法中获益，并且迄今为止，尚无已知的生物标志物可正确预测这些患者。最近，现代质谱方法已被用于确定代谢谱，尤其是脂质组学，它已成为肿瘤学和其他疾病研究的一个新领域。本研究旨在分析参加一项针对HNSCC器官保存的前瞻性、单中心、开放标签、非随机II期试验患者的唾液脂质谱。脂质分析采用基质辅助激光解吸/电离飞行时间质谱（MALDI-TOF-MS）进行。基于主成分分析和正交偏最小二乘判别分析的多变量统计分析应用于MALDI-TOF-MS数据，以可视化脂质谱之间的差异并识别潜在的生物标志物。结果有助于区分对治疗方案的完全缓解者和无反应者。总之，我们证明了一组脂质在接受器官保存方案的HNSCC患者的唾液中差异丰富，能够区分反应者和无反应者。这些结果表明脂质生物标志物在识别可能从该治疗中获益的患者方面具有潜在用途。此外，我们表明唾液检测可能在临床实践中常规使用，因为它是一种非侵入性的替代血液检测的方法，而且价格低廉且易于获取。
