Wang Xue-Ying, Zhang Ting, Guan Wei-Qun, Li Hua-Zhu, Lin Ling
Department of Stomatology, Fujian Medical University Union Hospital, Fuzhou, China.
General Dentistry, School and Hospital of Stomatology, Fujian Medical University, Fuzhou, China.
Front Oncol. 2021 Dec 22;11:771337. doi: 10.3389/fonc.2021.771337. eCollection 2021.
The aim of this study was to explore the lipidomic profiles of the CAL-27 human tongue cancer cell line and the human oral keratinocyte (HOK) cell line.
The lipidomic differences between the CAL-27 and the HOK cell lines were investigated using non-targeted high-performance liquid chromatography-mass spectrometry lipidomic analysis. The resulting data were then further mined bioinformatics analysis technology and metabolic pathway analysis was conducted in order to map the most affected metabolites and pathways in the two cell lines.
A total of 711 lipids were identified, including 403 glycerophospholipids (GPs), 147 glycerolipids, and 161 sphingolipids. Comparison of the enhanced MS (EMS) spectra of the two cell lines in positive and negative ionization modes showed the lipid compositions of HOK and CAL-27 cells to be similar. The expressions of most GP species in CAL-27 cells showed an increasing trend as compared with HOK, whereas a significant increase in phosphatidylcholine was observed ( < 0.05). Significant differences in the lipid composition between CAL-27 and HOK cells were shown as a heatmap. Through principal component analysis and orthogonal partial least squares discriminant analysis, noticeably clear separation trends and satisfactory clustering trends between groups of HOK and CAL-27 cells were identified. The numbers of specific lipid metabolites that could distinguish CAL-27 from HOK in positive and negative modes were 100 and 248, respectively. GP metabolism was the most significantly altered lipid metabolic pathway, with 4 metabolites differentially expressed in 39 hit products.
This study demonstrated the potential of using untargeted mass spectra and bioinformatics analysis to describe the lipid profiles of HOK and CAL-27 cells.
本研究旨在探索CAL-27人舌癌细胞系和人口腔角质形成细胞（HOK）系的脂质组学特征。
采用非靶向高效液相色谱-质谱脂质组学分析方法，研究CAL-27和HOK细胞系之间的脂质组差异。然后利用生物信息学分析技术对所得数据进行进一步挖掘，并进行代谢途径分析，以确定这两种细胞系中受影响最大的代谢物和途径。
共鉴定出711种脂质，包括403种甘油磷脂（GPs）、147种甘油脂和161种鞘脂。对两种细胞系在正离子和负离子模式下的增强质谱（EMS）谱图进行比较，结果显示HOK和CAL-27细胞的脂质组成相似。与HOK相比，CAL-27细胞中大多数GP种类的表达呈上升趋势，而磷脂酰胆碱的表达显著增加（<0.05）。通过热图展示了CAL-27和HOK细胞之间脂质组成存在显著差异。通过主成分分析和正交偏最小二乘法判别分析，发现HOK和CAL-27细胞组之间存在明显的分离趋势和令人满意的聚类趋势。在正离子和负离子模式下，能够区分CAL-27和HOK的特定脂质代谢物数量分别为100种和248种。GP代谢是脂质代谢途径中变化最显著的，在39种命中产物中有4种代谢物差异表达。
本研究证明了使用非靶向质谱和生物信息学分析来描述HOK和CAL-27细胞脂质谱的潜力。
