Lépine François, Milot Sylvain, Groleau Marie-Christine, Déziel Eric
INRS-Institut Armand-Frappier, 531 boulevard des Prairies, Laval, QC, Canada, H7V 1B7.
Methods Mol Biol. 2018;1673:49-59. doi: 10.1007/978-1-4939-7309-5_4.
High-performance liquid chromatography (HPLC) coupled in-line with mass spectrometry (MS) permits rapid and specific identification and quantification of N-acyl-L-homoserine lactones (AHLs) and 4-hydroxy-2-alkylquinolines (HAQs). We are presenting here methods for the analysis of these molecules directly from biological samples using LC/MS.
高效液相色谱(HPLC)与质谱(MS)联用,能够快速、特异性地鉴定和定量N-酰基-L-高丝氨酸内酯(AHLs)和4-羟基-2-烷基喹啉(HAQs)。我们在此介绍使用液相色谱/质谱联用技术直接从生物样品中分析这些分子的方法。