Hsu Phillip J, He Chuan
Department of Chemistry, Institute for Biophysical Dynamics, The University of Chicago, 929 E. 57th Street, Chicago, IL, USA.
Howard Hughes Medical Institute, The University of Chicago, Chicago, IL, USA.
Methods Mol Biol. 2018;1649:49-57. doi: 10.1007/978-1-4939-7213-5_3.
N -methyladenosine (mA) is the most abundant internal modification in eukaryotic mRNA, and is newly emerging as a key posttranscriptional mRNA regulator. Recent research has uncovered insight into the location and function of mA sites on a large scale, in part due to the transcriptome-wide identification of mA sites by high-throughput sequencing (mA-seq). Here, we present a protocol for mA-seq, which maps the mA methylome by affinity purification and sequencing.
N-甲基腺苷（mA）是真核生物信使核糖核酸（mRNA）中最丰富的内部修饰，并且作为一种关键的转录后mRNA调节剂正在崭露头角。最近的研究已经大规模地揭示了mA位点的位置和功能，部分原因是通过高通量测序（mA-seq）对转录组范围内的mA位点进行了鉴定。在这里，我们展示了一种用于mA-seq的方案，该方案通过亲和纯化和测序来绘制mA甲基化组图谱。
