Identifying the mA Methylome by Affinity Purification and Sequencing.

N -methyladenosine (mA) is the most abundant internal modification in eukaryotic mRNA, and is newly emerging as a key posttranscriptional mRNA regulator. Recent research has uncovered insight into the location and function of mA sites on a large scale, in part due to the transcriptome-wide identification of mA sites by high-throughput sequencing (mA-seq). Here, we present a protocol for mA-seq, which maps the mA methylome by affinity purification and sequencing.