Sun Juan, Chang Yan-Xiang, Niu Chun-Yan
1 Hospital of Shaanxi Nuclear Industry, Xianyang, China.
2 Xi'an Medical University, Xi'an, China.
Tumour Biol. 2017 Nov;39(11):1010428317726840. doi: 10.1177/1010428317726840.
The overexpression of soluble human leukocyte antigen-G is associated with malignant tumours. The purpose of our study was to detect soluble human leukocyte antigen-G concentrations in ascites and to evaluate the value of ascitic soluble human leukocyte antigen-G for the diagnosis of malignant ascites. Enzyme-linked immunosorbent assay was used to detect soluble human leukocyte antigen-G levels in 64 patients with malignant ascites and 30 patients with benign ascites. Receiver operating characteristic curves were used to evaluate the diagnostic efficacy of ascitic soluble human leukocyte antigen-G for the detection of malignant ascites. Ascitic soluble human leukocyte antigen-G levels were significantly higher in the malignant ascites group than in the benign ascites group (20.718 ± 3.215 versus 12.467 ± 3.678 µg/L, t = 7.425, p < 0.001). The area under the receiver operating characteristic curve for ascitic soluble human leukocyte antigen-G was 0.957 (95% confidence interval, 0.872-0.992). At a cut-off value of 19.60 µg/L, the sensitivity and specificity of ascitic soluble human leukocyte antigen-G were 87.5% (95% confidence interval, 71.0%-96.5%) and 100% (95% confidence interval, 88.4%-100%), respectively. With respect to area under the receiver operating characteristic curve, sensitivity and specificity, ascitic carcinoembryonic antigen (0.810, 68.75% and 83.33%, respectively) and carbohydrate antigen 19-9 (0.710, 65.63% and 70%, respectively) significantly differed (all p < 0.05). In malignant ascites that were cytology-negative and biopsy-positive, the rate of positivity for ascitic soluble human leukocyte antigen-G was 75%, which was higher than the corresponding rates for ascitic carcinoembryonic antigen (31.25%) and carbohydrate antigen 19-9 (6.25%; both p < 0.05). In conclusion, The detection of ascitic soluble human leukocyte antigen-G exhibited good performance for diagnosing malignant ascites, and particularly those that were cytology-negative and biopsy-positive.
可溶性人类白细胞抗原-G的过表达与恶性肿瘤相关。本研究的目的是检测腹水中可溶性人类白细胞抗原-G的浓度，并评估腹水可溶性人类白细胞抗原-G对恶性腹水诊断的价值。采用酶联免疫吸附测定法检测64例恶性腹水患者和30例良性腹水患者腹水中可溶性人类白细胞抗原-G的水平。采用受试者工作特征曲线评估腹水可溶性人类白细胞抗原-G对恶性腹水的诊断效能。恶性腹水组腹水中可溶性人类白细胞抗原-G水平显著高于良性腹水组（20.718±3.215对12.467±3.678μg/L，t=7.425，p<0.001）。腹水可溶性人类白细胞抗原-G的受试者工作特征曲线下面积为0.957（95%置信区间，0.872-0.992）。在临界值为19.60μg/L时，腹水可溶性人类白细胞抗原-G的敏感性和特异性分别为87.5%（95%置信区间，71.0%-96.5%）和100%（95%置信区间，88.4%-100%）。在受试者工作特征曲线下面积、敏感性和特异性方面，腹水癌胚抗原（分别为0.810、68.75%和83.33%）和糖类抗原19-9（分别为0.710、65.63%和7%）有显著差异（均p<0.05）。在细胞学检查阴性而活检阳性的恶性腹水中，腹水可溶性人类白细胞抗原-G的阳性率为75%，高于腹水癌胚抗原（31.25%）和糖类抗原19-9（6.25%；均p<0.05）的相应阳性率。总之，腹水可溶性人类白细胞抗原-G的检测在诊断恶性腹水中表现良好，尤其是对细胞学检查阴性而活检阳性的恶性腹水。
