Hamada Yui, Nakajima Mitsunari, Tsuzuki Kazuna, Amakura Yoshiaki, Yoshimura Morio, Okuyama Satoshi, Furukawa Yoshiko
Department of Pharmaceutical Pharmacology, College of Pharmaceutical Sciences, Matsuyama University, Matsuyama, Japan.
Int Arch Allergy Immunol. 2017;174(3-4):113-120. doi: 10.1159/000481094. Epub 2017 Nov 8.
In a previous study, we reported that interleukin-4 production was reduced in spleen cells of mice administered 3,5,6,7,8,3',4'-heptamethoxyflavone (HMF), which is a polymethoxyflavone found at high concentrations in the peel of various citrus fruits. In this study, we investigated the function of HMF on the growth of T cells cultured from the spleens of mice. HMF decreased the reduction of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) by anti-CD3/CD28 antibody-stimulated mouse spleen cells. HMF inhibited the activities of phosphodiesterase (PDE) enzymes prepared from bovine brain and human PDE4B and PDE3B enzymes. The cyclic AMP (cAMP) content in anti-CD3/CD28 antibody-stimulated spleen cells increased after HMF treatment in vitro. These results suggest that HMF inhibits T-cell growth and affects immune function via reduced PDE activity and increased cAMP content.
在之前的一项研究中，我们报道称，给小鼠施用3,5,6,7,8,3',4'-七甲氧基黄酮（HMF）后，其脾细胞中白细胞介素-4的产生减少，HMF是一种在各种柑橘类水果果皮中高浓度存在的多甲氧基黄酮。在本研究中，我们研究了HMF对从小鼠脾脏培养的T细胞生长的作用。HMF减少了抗CD3/CD28抗体刺激的小鼠脾细胞对3-(4,5-二甲基噻唑-2-基)-2,5-二苯基四氮唑溴盐（MTT）的还原。HMF抑制了从牛脑制备的磷酸二酯酶（PDE）以及人PDE4B和PDE3B酶的活性。体外经HMF处理后，抗CD3/CD28抗体刺激的脾细胞中的环磷酸腺苷（cAMP）含量增加。这些结果表明，HMF通过降低PDE活性和增加cAMP含量来抑制T细胞生长并影响免疫功能。
