Department of Biosystems Science and Engineering, ETH Zürich, Basel, Switzerland.
Nat Biotechnol. 2017 Dec;35(12):1202-1210. doi: 10.1038/nbt.4006. Epub 2017 Nov 13.
The bone marrow (BM) microenvironment contains many types of cells and molecules with roles in hematopoiesis, osteogenesis, angiogenesis and metabolism. The spatial distribution of the different bone and BM cell types remains elusive, owing to technical challenges associated with bone imaging. To map nonhematopoietic cells and structures in bone and BM, we performed multicolor 3D imaging of osteoblastic, vascular, perivascular, neuronal and marrow stromal cells, and extracellular-matrix proteins in whole mouse femurs. We analyzed potential interactions between cells and molecules on the basis of colocalization of markers. Our results shed light on the markers expressed by different osteolineage cell types; the heterogeneity of vascular and perivascular cells; the neural subtypes innervating marrow and bone; the diversity of stromal cells; and the distribution of extracellular-matrix components. Our complete imaging data set is available for download and can be used in research in bone biology, hematology, vascular biology, neuroscience and extracellular-matrix biology.
骨髓（BM）微环境包含多种在造血、成骨、血管生成和代谢中发挥作用的细胞和分子。由于与骨成像相关的技术挑战，不同骨和 BM 细胞类型的空间分布仍然难以捉摸。为了绘制骨和 BM 中非造血细胞和结构的图谱，我们对成骨细胞、血管、血管周细胞、神经元和骨髓基质细胞，以及整个小鼠股骨中的细胞外基质蛋白进行了多色 3D 成像。我们根据标记物的共定位分析了细胞和分子之间的潜在相互作用。我们的结果揭示了不同成骨细胞类型表达的标记物；血管和血管周细胞的异质性；支配骨髓和骨骼的神经亚型；基质细胞的多样性；以及细胞外基质成分的分布。我们完整的成像数据集可供下载，可用于骨生物学、血液学、血管生物学、神经科学和细胞外基质生物学的研究。
