Shaul Oren, Fanrazi-Kahana Michal, Meitav Omri, Pinhasi Gad A, Abookasis David
Appl Opt. 2017 Nov 10;56(32):8880-8886. doi: 10.1364/AO.56.008880.
Heat stress (HS) is a medical emergency defined by abnormally elevated body temperature that causes biochemical, physiological, and hematological changes. The goal of the present research was to detect variations in optical properties (absorption, reduced scattering, and refractive index coefficients) of mouse brain tissue during HS by using near-infrared (NIR) spatial light modulation. NIR spatial patterns with different spatial phases were used to differentiate the effects of tissue scattering from those of absorption. Decoupling optical scattering from absorption enabled the quantification of a tissue's chemical constituents (related to light absorption) and structural properties (related to light scattering). Technically, structured light patterns at low and high spatial frequencies of six wavelengths ranging between 690 and 970 nm were projected onto the mouse scalp surface while diffuse reflected light was recorded by a CCD camera positioned perpendicular to the mouse scalp. Concurrently to pattern projection, brain temperature was measured with a thermal camera positioned slightly off angle from the mouse head while core body temperature was monitored by thermocouple probe. Data analysis demonstrated variations from baseline measurements in a battery of intrinsic brain properties following HS.
热应激（HS）是一种由体温异常升高引起生化、生理和血液学变化的医疗急症。本研究的目的是通过使用近红外（NIR）空间光调制来检测热应激期间小鼠脑组织光学特性（吸收、约化散射和折射率系数）的变化。具有不同空间相位的近红外空间模式用于区分组织散射和吸收的影响。将光学散射与吸收解耦能够量化组织的化学成分（与光吸收有关）和结构特性（与光散射有关）。从技术上讲，在690至970nm范围内的六个波长的低空间频率和高空间频率的结构化光图案被投射到小鼠头皮表面，同时由垂直于小鼠头皮放置的CCD相机记录漫反射光。在图案投射的同时，用一个与小鼠头部稍有角度的热成像仪测量脑温，并用热电偶探头监测核心体温。数据分析表明，热应激后一系列内在脑特性与基线测量值存在差异。
