一种用于监测激酶活性的连续发光测定法：使用离散铕配合物指示ADP/ATP比率。
A continuous luminescence assay for monitoring kinase activity: signalling the ADP/ATP ratio using a discrete europium complex.
作者信息
Hewitt Sarah H, Parris Jonathan, Mailhot Romain, Butler Stephen J
机构信息
Department of Chemistry, Loughborough University, Epinal Way, Loughborough, LE11 3TU, UK.
出版信息
Chem Commun (Camb). 2017 Nov 23;53(94):12626-12629. doi: 10.1039/c7cc05887j.
PMID:29131206
Abstract
We report the application of a stable cationic europium complex [Eu.1]+ in a continuous-read luminescence assay for kinase activity. [Eu.1]+ binds reversibly to ATP and ADP in water, at neutral pH, in the presence of Mg ions, providing distinctive luminescence responses that permits the kinase-catalysed conversion of ATP to ADP to be monitored in real-time.
摘要
我们报道了一种稳定的阳离子铕配合物[Eu.1]+在激酶活性连续读取发光测定中的应用。[Eu.1]+在中性pH值、存在镁离子的水中与ATP和ADP可逆结合，提供独特的发光响应，从而能够实时监测激酶催化的ATP向ADP的转化。
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