Department of Cardiology, Hangzhou Red Cross Hospital, Hangzhou, Zhejiang, China.
Eur Rev Med Pharmacol Sci. 2017 Oct;21(20):4649-4653.
To investigate the expression and mechanism of plasma microRNA-21 in patients with ischemic cardiomyopathy (ICM).
56 cases of ICM patients were selected in our hospital from February 2010 to March 2016 as the observation group, and 60 cases of healthy patients were selected as control group. Real-time quantitative polymerase chain reaction (qRT-PCR) was used to detect the expression of microRNA-21 in two groups. Then, differences of the total cholesterol (TC), triglycerides (TG), left ventricular ejection fraction (LVEF), high and low density lipoprotein cholesterol (HDL-C, LDL-C), left ventricular end-diastolic volume (LVEDV), N terminal B type brain natriuretic peptide (NT-proBNP) and other clinical indicators of the two groups, were compared. The correlation between the plasma microRNA-21 level and the clinical indices was analyzed, and the value of microRNA-21 in the diagnosis and treatment of ICM was evaluated.
The levels of LDL-C, HDL-C and LVEF in the observation group were lower than those in the control group (p<0.05). Plasma microRNA-21, TG, NT-proBNP and LVEDV were higher than those in the control group; the difference was statistically significant (p<0.05). Logistic regression analysis showed that the plasma microRNA-21 level was positively correlated with NT-proBNP and LVEDV (p<0.05).
The expression of microRNA-21 in plasma of patients with ICM was significantly increased. And the expression of micro RNA-21 in plasma was positively correlated with NT-proBNP and LVEDV. Through the ventricular remodeling in ICM patients, it can be used as a new target for the diagnosis and treatment of ICM and a new biomarker for risk assessment.
探讨缺血性心肌病（ICM）患者血浆微小 RNA-21 的表达及机制。
选取我院 2010 年 2 月至 2016 年 3 月收治的 56 例 ICM 患者作为观察组，选择同期 60 例健康体检者作为对照组。采用实时定量聚合酶链反应（qRT-PCR）检测两组患者血浆微小 RNA-21 的表达水平，比较两组患者总胆固醇（TC）、三酰甘油（TG）、左心室射血分数（LVEF）、高低密度脂蛋白胆固醇（HDL-C、LDL-C）、左心室舒张末期容积（LVEDV）、N 末端 B 型脑钠肽前体（NT-proBNP）等临床指标的差异，分析血浆微小 RNA-21 水平与临床指标的相关性，并评价微小 RNA-21 在 ICM 诊断及治疗中的价值。
观察组患者 LDL-C、HDL-C、LVEF 水平低于对照组（P<0.05），血浆微小 RNA-21、TG、NT-proBNP、LVEDV 高于对照组，差异均有统计学意义（P<0.05）。Logistic 回归分析显示，血浆微小 RNA-21 水平与 NT-proBNP、LVEDV 呈正相关（P<0.05）。
ICM 患者血浆中微小 RNA-21 的表达明显升高，且血浆微小 RNA-21 表达与 NT-proBNP、LVEDV 呈正相关。通过对 ICM 患者心室重构的影响，可作为 ICM 诊断和治疗的新靶点，及风险评估的新生物标志物。
