Department of Gynecology, Zhangjiagang Hospital of Traditional Chinese Medicine Affiliated to Nanjing University of Chinese Medicine, Zhangjiagang, Jiangsu Province, China.
Eur Rev Med Pharmacol Sci. 2017 Oct;21(20):4542-4547.
MicroRNAs play critical roles in post-translational gene expression. The current study was to investigate the effects of miR-630 in epithelial ovarian cancer.
Thirty epithelial ovarian cancer tissue and thirty normal ovarian tissue samples were collected and were detected miR-630 expression level with qRT-PCR. MiR-630 mimics, inhibitors and negative controls were transfected into SKOV3 and Cell Counting Kit-8 (CCK-8) assay, and transwell experiment were performed to detect the proliferation rate and migration, respectively. The luciferase reporter assay was utilized to identify miR-630's target gene. Balb/c nude mice were utilized to verify the effect of miR-630 in vivo.
QRT-PCR showed a significantly high miR-630 expression in epithelial ovarian cancer relative to normal ovarian tissue. The miR-630 overexpression promoted epithelial ovarian cancer cell SKOV3 proliferation and migration. Krüppel-like factor 6 (KLF6) was predicted as the target of miR-630. In vivo study also verified that miR-630 overexpression stimulated ovarian cancer growth.
We propose that targeting miR-630 might be a promising therapeutic approach for ovarian cancer.
微小 RNA 在翻译后基因表达中发挥着关键作用。本研究旨在探讨 miR-630 在卵巢上皮性癌中的作用。
收集 30 例卵巢上皮性癌组织和 30 例正常卵巢组织标本，采用 qRT-PCR 检测 miR-630 的表达水平。转染 miR-630 模拟物、抑制剂和阴性对照至 SKOV3 细胞，通过细胞计数试剂盒-8（CCK-8）检测增殖率，Transwell 实验检测迁移能力。采用荧光素酶报告基因实验鉴定 miR-630 的靶基因。利用 Balb/c 裸鼠体内实验验证 miR-630 的作用。
qRT-PCR 显示 miR-630 在卵巢上皮性癌组织中表达显著升高。miR-630 的过表达促进了卵巢上皮性癌细胞 SKOV3 的增殖和迁移。Krüppel 样因子 6（KLF6）被预测为 miR-630 的靶基因。体内研究也验证了 miR-630 过表达刺激卵巢癌的生长。
我们提出靶向 miR-630 可能是治疗卵巢癌的一种有前途的方法。
