School of Liquor and Food Engineering, Guizhou Univ., Guiyang, 550025, China.
J Food Sci. 2017 Dec;82(12):2960-2967. doi: 10.1111/1750-3841.13975. Epub 2017 Nov 13.
Lactobacillus curvatus (SR6) and Lactobacillus paracasei (SR10-1) were assessed for their antioxidant activities and inoculated into sausages to investigate their effects on quality during fermentation. The results showed that L. curvatus SR6 had better DPPH• scavenging activity (59.67% ± 6.68%) and reducing power (47.31% ± 4.62%) and L. paracasei SR10-1 had better OH• scavenging activity (285.67% ± 2.00%) and anti-lipid peroxidation capacity (63.89% ± 0.93%). The superoxide dismutase activity of the cell culture fluid was greater than 47.00 U/mL, and the catalase activity of the cell-free extracts was greater than 1.00 U/mL. In the sausage model, lactic acid bacteria rapidly became the dominant microflora and reduced the moisture content, water activity, nitrite, and pH. The bacteria significantly enhanced the antioxidant activity of the sausage extracts, which improved the sensory characteristics and safety of the sausages. These results illustrate that both strains have excellent antioxidant activities and can be used as antioxidant starters in fermented meat products.
The study illustrated the antioxidant and antioxidase activities of Lactobacillus curvatus SR6 and Lactobacillus paracasei SR10-1 and demonstrated the changes in the quality characteristics and antioxidant activities of fermented sausages. The findings provide valuable information for the meat industry on the application of functional starters in fermented meat products.
评估了弯曲乳杆菌(SR6)和副干酪乳杆菌(SR10-1)的抗氧化活性,并将其接种到香肠中,以研究其在发酵过程中对质量的影响。结果表明,弯曲乳杆菌 SR6 具有更好的 DPPH•清除活性(59.67%±6.68%)和还原能力(47.31%±4.62%),而副干酪乳杆菌 SR10-1 具有更好的 OH•清除活性(285.67%±2.00%)和抗脂质过氧化能力(63.89%±0.93%)。细胞培养液的超氧化物歧化酶活性大于 47.00 U/mL,细胞无细胞提取物的过氧化氢酶活性大于 1.00 U/mL。在香肠模型中,乳酸菌迅速成为优势菌群,降低了水分含量、水分活度、亚硝酸盐和 pH 值。细菌显著增强了香肠提取物的抗氧化活性,改善了香肠的感官特性和安全性。这些结果表明,这两种菌株都具有出色的抗氧化活性,可作为发酵肉制品中的抗氧化剂。
该研究说明了弯曲乳杆菌 SR6 和副干酪乳杆菌 SR10-1 的抗氧化和抗氧化酶活性,并证明了发酵香肠质量特性和抗氧化活性的变化。研究结果为肉类行业在发酵肉制品中应用功能性发酵剂提供了有价值的信息。