Angle Orthod. 2018 Mar;88(2):179-186. doi: 10.2319/051117-328.1. Epub 2017 Nov 13.
To analyze histologic findings of bone remodeling at the time of surgery and 90 days later at corticotomized and noncorticotomized sites.
Eight adult patients with bilaterally inclined mandibular molars were included in this split-mouth study. Corticotomy was performed on the test side, and bone was harvested on both mandibular hemi-arches. Bone blocks were shipped to Forsyth Institute lab (Boston, Mass). After surgery, cantilevers were activated to upright inclined mandibular molars on both sides. Approximately 90 days after surgery, new mandibular bone blocks were harvested from test and control sites. Histologic analyses were performed on both groups (test and control sides) at two time points: baseline (T0) and 90 days after surgery (T90). Bone blocks were stained with hematoxylin and eosin, and Masson's trichrome. The protocols enabled screening of new bone formation and collagen in mineralized and nonmineralized connective tissue. Outcomes were compared using a clustered generalized estimating equations model Results: The test group at T90 exhibited an increase of 16% of primary bone ( P < .05), an increase of 6.6 in osteocytes quantity ( P = .039), and an increase of 4.06 in reversal lines quantity ( P = .924). Both groups exhibited a decrease in the inorganic proportion.
The intentional bone injury performed in adult patients undergoing orthodontic treatment can be considered temporary and reversible. Evidence indicates that baseline bone characteristics are unaltered.
分析手术时和皮质切开及非皮质切开部位 90 天后骨改建的组织学发现。
本研究为分侧研究，纳入 8 例双侧下颌倾斜磨牙的成年患者。在试验侧进行皮质切开，在下颌半弓的两侧采集骨块。将骨块运往福赛思研究所（马萨诸塞州波士顿）的实验室。手术后，在两侧均使用支抗将倾斜的下颌磨牙直立。术后约 90 天，从试验组和对照组采集新的下颌骨块。两组（试验组和对照组）在两个时间点（基线 T0 和术后 90 天 T90）进行组织学分析：基线（T0）和术后 90 天（T90）。骨块用苏木精和伊红、马松三色染色。该方案可筛选新骨形成和矿化及非矿化结缔组织中的胶原。使用聚类广义估计方程模型比较结果：T90 时试验组原发性骨增加 16%（P<0.05），骨细胞数量增加 6.6（P=0.039），反转线数量增加 4.06（P=0.924）。两组的无机比例均下降。
接受正畸治疗的成年患者中进行的有意骨损伤可被认为是暂时和可逆的。证据表明基线骨特征未改变。
