Medeiros Kelsea L, Lucio-Forster Araceli, Bowman Dwight Douglas
From the Department of Microbiology and Immunology, College of Veterinary Medicine, Cornell University, Ithaca, New York.
J Am Anim Hosp Assoc. 2018 Jan/Feb;54(1):36-45. doi: 10.5326/JAAHA-MS-6549. Epub 2017 Nov 13.
Two new devices (OT, ST), were recently introduced for the recovery of parasite eggs and cysts for microscopic examination. These devices, two stationary flotation devices, and a standard double-centrifugal sugar-flotation were compared using common flotation solutions and methods recommended by the manufacturers for the recovery of hookworm, ascaridoid, and whipworm eggs from companion animal fecal samples. Additionally, the recovery of Giardia cysts in the OT device using a zinc sulfate versus sodium nitrate solution was evaluated. Double-centrifugal sugar-flotation (1.30 specific gravity) was the most sensitive method for the recovery of the nematode eggs from feces of companion animals. Overall, centrifugation increased the recovery of eggs as compared with standing flotation methods, with the ST performing equivalently to the OT. Although these more recently introduced tests have good sensitivities for the nematodes tested, egg recovery was routinely markedly less than that achieved by standard double-centrifugal sugar-flotation, and false-negatives did occur. Still, the OT and ST generally have increased recoveries over the two standing flotation devices, and are significantly better than these for the recovery of ascaridoid and whipworm eggs from dog and cat samples. Zinc sulfate (1.18 specific gravity) is recommended for the recovery of Giardia cysts when using the OT device.
最近推出了两种新设备（OT、ST），用于收集寄生虫卵和囊肿以进行显微镜检查。使用常用的浮选溶液和制造商推荐的方法，将这两种设备（两种静态浮选设备）与标准的双离心糖浮选法进行比较，以从伴侣动物粪便样本中收集钩虫卵、蛔虫卵和鞭虫卵。此外，还评估了在OT设备中使用硫酸锌与硝酸钠溶液回收贾第虫囊肿的情况。双离心糖浮选法（比重1.30）是从伴侣动物粪便中回收线虫卵最灵敏的方法。总体而言，与静置浮选法相比，离心法提高了虫卵的回收率，ST的表现与OT相当。尽管这些最近推出的检测方法对所检测的线虫具有良好的灵敏度，但虫卵回收率通常明显低于标准双离心糖浮选法，并且确实会出现假阴性结果。不过，OT和ST通常比两种静置浮选设备的回收率更高，并且在从狗和猫的样本中回收蛔虫卵和鞭虫卵方面明显优于它们。使用OT设备时，推荐使用硫酸锌（比重1.18）来回收贾第虫囊肿。
