Guangdong Key Laboratory of Biomedical Engineering, School of Materials Science and Engineering, South China University of Technology, Guangzhou 510641, PR China.
Guangdong Key Laboratory of Biomedical Engineering, School of Materials Science and Engineering, South China University of Technology, Guangzhou 510641, PR China.
Colloids Surf B Biointerfaces. 2018 Feb 1;162:1-7. doi: 10.1016/j.colsurfb.2017.11.020. Epub 2017 Nov 8.
Beta-tricalcium phosphate (β-TCP) nanocoatings, which can be analysed using a quartz crystal microbalance with dissipation technique (QCM-D), were fabricated on a gold surface by electrophoretic deposition. The influences of electric field intensity and electrophoresis time were investigated. The adsorption behaviours of bovine serum albumin (BSA) and lysozyme (LSZ) on Au and β-TCP surfaces were observed in real time by QCM-D. The homogeneous β-TCP nanocoating with moderately sized particles on gold surface was fabricated at 25V/cm for 5min, and it met the requirements for the QCM-D experiment. The adsorption behaviour of BSA was different from that of LSZ, which was caused by the differences of protein properties. The adsorption quantity of BSA on a β-TCP surface was higher than that on a gold surface. However, the adsorption amount of LSZ on a β-TCP surface was lower than that on a gold surface. The electrostatic force was the major factor affecting the adsorption quantities of BSA and LSZ on Au and β-TCP surfaces based on the investigation of various factors. The findings reported here will be useful for understanding the mechanism of the interaction between biomaterials and proteins.
β-磷酸三钙（β-TCP）纳米涂层可通过石英晶体微天平耗散技术（QCM-D）进行分析，通过电泳沉积在金表面上制备。研究了电场强度和电泳时间的影响。通过 QCM-D 实时观察牛血清白蛋白（BSA）和溶菌酶（LSZ）在 Au 和β-TCP 表面上的吸附行为。在 25V/cm 下电泳 5min 可在金表面上制备具有适度粒径的均匀β-TCP 纳米涂层，满足 QCM-D 实验的要求。BSA 的吸附行为与 LSZ 不同，这是由于蛋白质性质的差异所致。BSA 在β-TCP 表面上的吸附量高于在金表面上的吸附量。然而，LSZ 在β-TCP 表面上的吸附量低于在金表面上的吸附量。基于对各种因素的研究，静电引力是影响 BSA 和 LSZ 在 Au 和β-TCP 表面上吸附量的主要因素。本研究结果将有助于理解生物材料与蛋白质相互作用的机制。
