Zhu Xiangdong, Fan Hongsong, Li Dongxiao, Xiao Yumei, Zhang Xingdong
National Engineering Research Center for Biomaterials, Sichuan University, Chengdu, China.
J Biomed Mater Res B Appl Biomater. 2007 Jul;82(1):65-73. doi: 10.1002/jbm.b.30706.
An investigation on the relationship between protein adsorption and zeta potentials of a biphasic calcium phosphate (BCP) ceramic was carried out. Zeta potentials of the BCP ceramic particles were measured at various aqueous solutions. Bovine serum album (BSA) and its competitive adsorption with lysozyme (LSZ) on BCP were investigated using conventional protein adsorption and polyacrylamide gel electrophoresis (PAGE) methods. The results showed that zeta potential and the amount of adsorbed BSA were both influenced by pH, ionic strength, Ca2+ and PO4(3-) concentrations in the buffers. The variation tendencies of BSA adsorption were consistent with that of zeta potentials to some extent. The co-adsorption of BSA and LSZ on BCP was confirmed by the PAGE gel pattern. The semi-quantitative analysis for the detected protein bands proved that LSZ had higher affinity for BCP than BSA and would preferentially bind to the surface. Electrostatic interaction played an important role in protein adsorption on the surface of the BCP ceramic particles.
对双相磷酸钙（BCP）陶瓷的蛋白质吸附与zeta电位之间的关系进行了研究。在各种水溶液中测量了BCP陶瓷颗粒的zeta电位。使用传统的蛋白质吸附和聚丙烯酰胺凝胶电泳（PAGE）方法研究了牛血清白蛋白（BSA）及其与溶菌酶（LSZ）在BCP上的竞争性吸附。结果表明，zeta电位和BSA的吸附量均受缓冲液中pH、离子强度、Ca2+和PO4(3-)浓度的影响。BSA吸附的变化趋势在一定程度上与zeta电位的变化趋势一致。通过PAGE凝胶图谱证实了BSA和LSZ在BCP上的共吸附。对检测到的蛋白条带的半定量分析证明，LSZ对BCP的亲和力高于BSA，并且会优先结合到表面。静电相互作用在蛋白质吸附到BCP陶瓷颗粒表面的过程中起重要作用。
