Sallon C, Soulet D, Tremblay Y
Axe reproduction, santé de la mère et de l'enfant, centre de recherche du CHU de Québec, Québec, QC, Canada; Centre de recherche en reproduction, développement et santé intergénérationnelle (CRDSI), faculté de médecine, université Laval, Québec, QC, Canada.
Axe neuroscience, centre de recherche du CHU de Québec, Québec, QC, Canada; Faculté de pharmacie, université Laval, Québec, QC, Canada.
Rev Mal Respir. 2017 Dec;34(10):1072-1084. doi: 10.1016/j.rmr.2017.09.003. Epub 2017 Nov 11.
Weibel's research has shown that any alteration of the pulmonary structure has effects on function. This demonstration required a quantitative analysis of lung structures called morphometry. This is possible thanks to stereology, a set of methods based on principles of geometry and statistics. His work has helped to better understand the morphological harmony of the lung, which is essential for its proper functioning. An imbalance leads to pathophysiology such as chronic obstructive pulmonary disease in adults and bronchopulmonary dysplasia in neonates. It is by studying this imbalance that new therapeutic approaches can be developed. These advances are achievable only through morphometric analytical methods, which are increasingly precise and focused, in particular thanks to the high-throughput automation of these methods. This review makes a comparison between an automated method that we developed in the laboratory and semi-manual methods of morphometric analyzes. The automation of morphometric measurements is a fundamental asset in the study of pulmonary pathophysiology because it is an assurance of robustness, reproducibility and speed. This tool will thus contribute significantly to the acceleration of the race for the development of new drugs.
魏贝尔的研究表明，肺部结构的任何改变都会对功能产生影响。这一证明需要对称为形态计量学的肺部结构进行定量分析。这得益于体视学得以实现，体视学是一组基于几何和统计原理的方法。他的工作有助于更好地理解肺部的形态和谐，这对其正常功能至关重要。失衡会导致病理生理学状况，如成人的慢性阻塞性肺疾病和新生儿的支气管肺发育不良。正是通过研究这种失衡，才能够开发出新的治疗方法。这些进展只有通过形态计量分析方法才能实现，这些方法越来越精确且针对性强，特别是由于这些方法的高通量自动化。本综述对我们在实验室开发的一种自动化方法与形态计量分析的半手动方法进行了比较。形态计量测量的自动化是肺部病理生理学研究的一项基本资产，因为它保证了稳健性、可重复性和速度。因此，这个工具将极大地有助于加速新药研发的竞争。
