Department of Neurosurgery, The Affiliated Hospital of Qingdao University, Qingdao, Shandong 266003, China.
Department of Neurosurgery, Xiangya Hospital, Central South University, Changsha, Hunan 410008, China.
Biosci Rep. 2018 Nov 20;38(6). doi: 10.1042/BSR20171043. Print 2018 Dec 21.
In certain surgical procedures, sacrificing the superior petrosal vein (SPV) is required. Previous studies have reported transient cerebellar edema, venous infarction, or hemorrhage that might occur after sectioning of the SPV. The present study investigated the pathophysiological changes in cerebellum and brain stem after SPV sacrifice. Rabbits were divided into the operation group where the SPV was sacrificed and the control group where the SPV remained intact. Each group was further subdivided into 4, 8, 12, 24, 48, and 72 h groups which represented the time period from sacrificing of the SPV to killing of the rabbits. The water content (WC), Na content, K content, and pathophysiological changes in cerebellum and brain stem tissue were measured. In comparison with the control, the WC and Na content of cerebellar tissue were increased in the 4, 8, 12, and 24 h operation subgroups (<0.05), but only increased in the 4-h subgroup of the brain stem tissue (<0.05). The K content of the cerebellar tissue decreased in the 4, 8, 12, and 24 h operation subgroups (<0.05) but only decreased in the 4-h subgroup of brain stem tissue (<0.05). Nissl staining and TEM demonstrated that cerebellar edema occurred in the 4, 8, 12, and 24 h operation subgroups but not in the 48- and 72-h subgroups. Brain stem edema occurred in the 4-h operation subgroup. In summary, cerebellum and brain stem edema can be observed at different time points after sacrificing of the SPV in the rabbit model.
在某些外科手术中，需要牺牲岩上窦（SPV）。先前的研究报告称，在切开 SPV 后可能会出现短暂的小脑水肿、静脉梗死或出血。本研究探讨了 SPV 牺牲后小脑和脑干的病理生理变化。兔子被分为手术组（SPV 被牺牲）和对照组（SPV 保持完整）。每组进一步分为 4、8、12、24、48 和 72 h 组，代表从牺牲 SPV 到杀死兔子的时间。测量了小脑和脑干组织的含水量（WC）、Na 含量、K 含量和病理生理变化。与对照组相比，手术组小脑组织的 WC 和 Na 含量在 4、8、12 和 24 h 亚组中增加（<0.05），但仅在脑干组织的 4 h 亚组中增加（<0.05）。小脑组织的 K 含量在 4、8、12 和 24 h 手术亚组中减少（<0.05），但仅在脑干组织的 4 h 亚组中减少（<0.05）。尼氏染色和 TEM 显示，小脑水肿发生在 4、8、12 和 24 h 手术亚组中，但不在 48 和 72 h 亚组中。脑干部水肿发生在 4 h 手术亚组中。总之，在兔模型中，SPV 牺牲后可以在不同时间点观察到小脑和脑干部水肿。
