Department of Neurosurgery, the Affiliated Hospital of Qingdao University, Qingdao, Shandong 266003, China; Department of Physiology, Shandong Key Laboratory of Pathogenesis and Prevention of Neurological Disorders and State Key Disciplines: Physiology, School of basic Medicine, Qingdao University, Qingdao, Shandong 266071, China.
Department of Physiology, Shandong Key Laboratory of Pathogenesis and Prevention of Neurological Disorders and State Key Disciplines: Physiology, School of basic Medicine, Qingdao University, Qingdao, Shandong 266071, China.
J Chem Neuroanat. 2020 Jul;106:101791. doi: 10.1016/j.jchemneu.2020.101791. Epub 2020 Apr 25.
Cerebral edema after brain surgery remains a life-threatening complication in the clinic. For a better operating field view, superior petrosal vein (SPV) can be easily damaged during neurosurgery. SPV sacrifice may sometimes be inevitable in clinic. However, the safety of SPV sacrifice is still a controversial question. Whether petrosal vein injury has an effect on cerebral edema after brain surgery is still unknown. In this study, rabbits were divided into two groups. The rabbits in the surgery group underwent petrosal vein sacrifice. The control group was subjected to sham surgery. Cerebellum and brain stem tissues were collected at 4 h, 8 h, 12 h, 24 h, 48 h and 72 h post-surgery. The superoxidase dismutase (SOD) activity and expression of malondialdehyde (MDA) were tested in the collected samples. Quantitiative real time polymerase chain reaction and immunohistochemistry were used to detect the mRNA and protein levels, respectively, of aquaporin 4 (AQP4) in the tissue samples. Compared to the control sham group, the activity of SOD and MDA expression in cerebellum was decreased and increased, respectively, at 4 h, 8 h, 12 h and 24 h post-, surgery The SOD activity and expression of MDA in brain stem was decreased and increased, respectively, only in 4 h after surgery, compared with control group. The mRNA and protein levels of AQP4 were increased in cerebellum at 4 h, 8 h, 12 h and 24 h after surgery, but in the brain stem, the levels were increased only at 4 h after surgery compared with sham group. Our results thus show that SPV sacrifice influences oxidative stress and the expression of AQP4 in cerebellum and brain stem of rabbits; highlighting the importance of protecting the petrosal vein during neurosurgery.
脑手术后脑水肿仍然是临床中危及生命的并发症。为了获得更好的手术视野，岩上静脉（SPV）在神经外科手术中很容易受损。在临床实践中，SPV 的牺牲有时可能是不可避免的。然而，SPV 牺牲的安全性仍然是一个有争议的问题。岩上静脉损伤是否对脑手术后的脑水肿有影响仍不清楚。在这项研究中，兔子被分为两组。手术组行岩上静脉牺牲，对照组行假手术。术后 4 h、8 h、12 h、24 h、48 h 和 72 h 采集小脑和脑干组织。检测收集样本中超氧化物歧化酶（SOD）的活性和丙二醛（MDA）的表达。采用定量实时聚合酶链反应和免疫组织化学法分别检测组织样本中水通道蛋白 4（AQP4）的 mRNA 和蛋白水平。与对照组相比，手术组小脑 SOD 活性在术后 4 h、8 h、12 h 和 24 h 时降低，MDA 表达增加；脑桥 SOD 活性在术后 4 h 时降低，MDA 表达在术后 4 h 时增加，与对照组相比。AQP4 的 mRNA 和蛋白水平在小脑的表达在术后 4 h、8 h、12 h 和 24 h 时增加，但在脑桥中，与假手术组相比，仅在术后 4 h 时增加。因此，我们的研究结果表明，SPV 牺牲影响了兔小脑和脑桥的氧化应激和 AQP4 的表达；强调了在神经外科手术中保护岩上静脉的重要性。
