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作为大规模快速生物多样性评估项目的一部分,针对鳞翅目DNA条形码的简化采集与制备方案,以印度尼西亚生物多样性发现与信息系统(IndoBioSys)为例。

A streamlined collecting and preparation protocol for DNA barcoding of Lepidoptera as part of large-scale rapid biodiversity assessment projects, exemplified by the Indonesian Biodiversity Discovery and Information System (IndoBioSys).

作者信息

Schmidt Olga, Hausmann Axel, Cancian de Araujo Bruno, Sutrisno Hari, Peggie Djunijanti, Schmidt Stefan

机构信息

SNSB - Zoologische Staatssammlung München, Munich, Germany.

Division of Zoology, Research Center for Biology, Indonesian Institute of Sciences, Cibinong, Indonesia.

出版信息

Biodivers Data J. 2017 Oct 31(5):e20006. doi: 10.3897/BDJ.5.e20006. eCollection 2017.

DOI:10.3897/BDJ.5.e20006
PMID:29134041
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5676197/
Abstract

Here we present a general collecting and preparation protocol for DNA barcoding of Lepidoptera as part of large-scale rapid biodiversity assessment projects, and a comparison with alternative preserving and vouchering methods. About 98% of the sequenced specimens processed using the present collecting and preparation protocol yielded sequences with more than 500 base pairs. The study is based on the first outcomes of the Indonesian Biodiversity Discovery and Information System (IndoBioSys). IndoBioSys is a German-Indonesian research project that is conducted by the Museum für Naturkunde in Berlin and the Zoologische Staatssammlung München, in close cooperation with the Research Center for Biology - Indonesian Institute of Sciences (RCB-LIPI, Bogor).

摘要

在此,我们展示了一种用于鳞翅目DNA条形码分析的通用采集与制备方案,作为大规模快速生物多样性评估项目的一部分,并与其他保存和凭证方法进行了比较。使用本采集与制备方案处理的测序标本中,约98%产生了超过500个碱基对的序列。该研究基于印度尼西亚生物多样性发现与信息系统(IndoBioSys)的首批成果。IndoBioSys是一个德印研究项目,由柏林自然博物馆和慕尼黑国家动物标本馆与印度尼西亚科学院生物学研究中心(RCB-LIPI,茂物)密切合作开展。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e90f/5676197/c5d534d505ae/bdj-05-e20006-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e90f/5676197/dff0e9ed6d32/bdj-05-e20006-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e90f/5676197/77f12f79edcf/bdj-05-e20006-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e90f/5676197/c5d534d505ae/bdj-05-e20006-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e90f/5676197/dff0e9ed6d32/bdj-05-e20006-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e90f/5676197/77f12f79edcf/bdj-05-e20006-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e90f/5676197/c5d534d505ae/bdj-05-e20006-g003.jpg

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