Cancian de Araujo Bruno, Schmidt Stefan, Schmidt Olga, von Rintelen Thomas, von Rintelen Kristina, Floren Andreas, Ubaidillah Rosichon, Peggie Djunijanti, Balke Michael
SNSB-Zoologische Staatssammlung München, Munich, Germany SNSB-Zoologische Staatssammlung München Munich Germany.
Museum für Naturkunde, Berlin, Germany Museum für Naturkunde Berlin Germany.
Biodivers Data J. 2019 Jan 15(7):e31432. doi: 10.3897/BDJ.7.e31432. eCollection 2019.
We present the results of a DNA barcoding pipeline that was established as part of the German-Indonesian IndobioSys project - Indonesian Biodiversity Information System. Our data release provides the first large-scale diversity assessment of Indonesian coleoptera obtained by canopy fogging. The project combined extensive fieldwork with databasing, DNA barcode based species delineation and the release of results in collaboration with Indonesian counterparts, aimed at supporting further analyses of the data. Canopy fogging on 28 trees was undertaken at two different sites, Cikaniki and Gunung Botol, in the south-eastern area of the Gunung Halimun-Salak National Park in West Java, Indonesia. In total, 7,447 specimens of Coleoptera were processed, of which 3,836 specimens produced DNA barcode sequences that were longer than 300 bp. A total of 3,750 specimens were assigned a Barcode Index Number (BIN), including 2,013 specimens from Cikaniki and 1,737 specimens from Gunung Botol. The 747 BINs, that were obtained, represented 39 families of Coleoptera. The distribution of specimens with BINs per tree was quite heterogeneous in both sites even in terms of the abundance of specimens or diversity of BINs. The specimen distribution per taxon was heterogeneous as well. Some 416 specimens could not be identified to family level, corresponding to 72 BINs that lack a family level identification. The data have shown a large heterogeneity in terms of abundance and distribution of BINs between sites, trees and families of Coleoptera. From the total of 747 BINs that were recovered, 421 (56%) are exclusive from a single tree. Although the two study sites were in close proximity and separated by a distance of only about five kilometres, the number of shared BINs between sites is low, with 81 of the 747 BINs. With this data release, we expect to shed some light on the largely hidden diversity in the canopy of tropical forests in Indonesia and elsewhere.
我们展示了作为德国 - 印度尼西亚IndobioSys项目（印度尼西亚生物多样性信息系统）一部分而建立的DNA条形码流程的结果。我们的数据发布提供了通过树冠喷雾获得的印度尼西亚鞘翅目昆虫的首次大规模多样性评估。该项目将广泛的实地工作与数据库建设、基于DNA条形码的物种划分以及与印度尼西亚同行合作发布结果相结合，旨在支持对数据的进一步分析。在印度尼西亚西爪哇省哈利蒙 - 萨拉克国家公园东南部地区的两个不同地点，即芝卡尼基和博托尔山，对28棵树进行了树冠喷雾。总共处理了7447个鞘翅目标本，其中3836个标本产生了长度超过300 bp的DNA条形码序列。总共3750个标本被分配了条形码索引号（BIN），其中包括来自芝卡尼基的2013个标本和来自博托尔山的1737个标本。获得的747个BIN代表了鞘翅目的39个科。在两个地点，每棵树上具有BIN的标本分布在标本数量或BIN多样性方面都相当不均匀。每个分类单元的标本分布也不均匀。约416个标本无法鉴定到科级水平，对应于72个缺乏科级鉴定的BIN。数据显示，在鞘翅目的不同地点、树木和科之间，BIN的丰度和分布存在很大的不均匀性。在总共回收的747个BIN中，有421个（56%）仅来自一棵树。尽管两个研究地点相距很近，仅相隔约5公里，但地点之间共享的BIN数量很少，在747个BIN中只有81个。通过此次数据发布，我们期望能够揭示印度尼西亚及其他地区热带森林树冠层中很大程度上隐藏的多样性。
