Arocena Miguel, Rajnicek Ann M, Collinson Jon Martin
Institute of Medical Sciences, University of Aberdeen, Aberdeen, UK.
Facultad de Ciencias, UDELAR, Montevideo, Uruguay.
R Soc Open Sci. 2017 Oct 4;4(10):170625. doi: 10.1098/rsos.170625. eCollection 2017 Oct.
The intricate patterns of cell migration that are found throughout development are generated through a vast array of guidance cues. Responding integratively to distinct, often conflicting, migratory signals is probably crucial for cells to reach their correct destination. is a master transcription factor with key roles in neural development that include the control of cell migration. In this study, we have investigated the ability of cells derived from cortical neurospheres from wild-type (WT) and mouse embryos to integrate diverging guidance cues. We used two different cues, either separately or in combination: substratum nanogrooves to induce contact guidance, and electric fields (EFs) to induce electrotaxis. In the absence of an EF, both WT and cells aligned and migrated parallel to grooves, and on a flat substrate both showed marked electrotaxis towards the cathode. When an EF was applied in a perpendicular orientation to grooves, WT cells responded significantly to both cues, migrating in highly oblique trajectories in the general direction of the cathode. However, cells had an impaired response to both cues simultaneously. Our results demonstrate that these neurosphere derived cells have the capacity to integrate diverging guidance cues, which requires function.
在整个发育过程中发现的复杂细胞迁移模式是通过大量的引导线索产生的。对不同的、通常相互冲突的迁移信号进行综合响应可能对细胞到达其正确目的地至关重要。 是一种在神经发育中起关键作用的主转录因子，其作用包括控制细胞迁移。在本研究中，我们研究了来自野生型（WT）和 小鼠胚胎的皮质神经球的细胞整合不同引导线索的能力。我们分别或组合使用两种不同的线索：基质纳米凹槽以诱导接触引导，以及电场（EFs）以诱导电趋性。在没有EF的情况下，WT细胞和 细胞都与凹槽对齐并平行于凹槽迁移，并且在平坦基板上两者都显示出明显的向阴极电趋性。当EF以垂直于凹槽的方向施加时，WT细胞对两种线索都有显著反应，沿阴极的大致方向以高度倾斜的轨迹迁移。然而， 细胞同时对两种线索的反应受损。我们的结果表明，这些源自神经球的细胞具有整合不同引导线索的能力，这需要 功能。
