Wu Liping, Xie Jinshan, Li Tan, Mai Zihao, Wang Lu, Wang Xiaoping, Chen Tongsheng
MOE Key Laboratory of Laser Life Science, College of Biophotonics, South China Normal University, Guangzhou, Guangdong, People's Republic of China.
Department of Pain Management, The First Affiliated Hospital of Jinan University, Guangzhou, Guangdong, People's Republic of China.
R Soc Open Sci. 2017 Oct 25;4(10):170822. doi: 10.1098/rsos.170822. eCollection 2017 Oct.
We recently developed a polyethylenimine (PEI) and polyethylene glycol (PEG) dual-functionalized reduced graphene oxide (GO) (PEG-nrGO-PEI, RGPP) for high-efficient gene delivery in HepG2 and Hela cell lines. To evaluate the feasibility and applicability of RGPP as a gene delivery carrier, we here assessed the transfection efficiency of RGPP on gene plasmids and siRNA in 11 different cell lines. Commercial polyalkyleneimine cation transfection reagent (TR) was used as comparison. In HepG2 cells, RGPP exhibited much stronger delivery ability for siRNA and large size plasmids than TR. For green fluorescent protein (GFP) plasmid, RGPP showed about 47.1% of transfection efficiency in primary rabbit articular chondrocytes, and about 27% of transfection efficiency in both SH-SY5Y and A549 cell lines. RGPP exhibited about 37.2% of GFP plasmid transfection efficiency in EMT6 cells and about 26.0% of GFP plasmid transfection efficiency in LO2 cells, but induced about 33% of cytotoxicity in both cell lines. In 4T1 and H9C2 cell lines, RGPP had less than 10% of GFP plasmid transfection efficiency. Collectively, RGPP is a potential nano-carrier for high-efficiency gene delivery, and needs to be further optimized for different cell lines.
我们最近开发了一种聚乙烯亚胺（PEI）和聚乙二醇（PEG）双功能化的还原氧化石墨烯（GO）（PEG-nrGO-PEI，RGPP），用于在HepG2和Hela细胞系中进行高效基因递送。为了评估RGPP作为基因递送载体的可行性和适用性，我们在此评估了RGPP对11种不同细胞系中基因质粒和小干扰RNA（siRNA）的转染效率。使用商业聚亚烷基胺阳离子转染试剂（TR）作为对照。在HepG2细胞中，RGPP对siRNA和大尺寸质粒的递送能力比TR强得多。对于绿色荧光蛋白（GFP）质粒，RGPP在原代兔关节软骨细胞中的转染效率约为47.1%，在SH-SY5Y和A549细胞系中的转染效率约为27%。RGPP在EMT6细胞中的GFP质粒转染效率约为37.2%，在LO2细胞中的GFP质粒转染效率约为26.0%，但在这两种细胞系中均诱导了约33%的细胞毒性。在4T1和H9C2细胞系中，RGPP的GFP质粒转染效率低于10%。总的来说，RGPP是一种用于高效基因递送的潜在纳米载体，需要针对不同细胞系进一步优化。
