Zhang Liming, Wang Zunliang, Lu Zhuoxuan, Shen He, Huang Jie, Zhao Qinghuan, Liu Min, He Nongyue, Zhang Zhijun
Division of Nanobiomedicine, Suzhou Institute of Nano-tech and Nano-bionics, Chinese Academy of Sciences, 398 Ruoshui Road, Suzhou, 215123, P. R. China.
J Mater Chem B. 2013 Feb 14;1(6):749-755. doi: 10.1039/c2tb00096b. Epub 2012 Nov 28.
Single stranded ribonucleic acid (ssRNA) acts as a probe, antisense (AS), miRNA analog and inhibitor, and is promising for gene therapy and molecular diagnosis. However, free ssRNA exhibits poor cellular uptake due to its negative charges, and enzyme instability, which have largely limited the practical applications of ssRNA in biomedicine. To address these issues, we have developed a PEGylated reduced graphene oxide (PEG-RGO) nanovector for efficient delivery of ssRNA. We have demonstrated that PEG-RGO exhibits superior ssRNA loading and delivery capability, compared to the widely studied PEGylated graphene oxide (PEG-GO). Computational simulation further suggested that PEG-RGO binds ssRNA much stronger than PEG-GO, consistent with the experimental results. These results will have implications in designing RGO-based biocompatible and efficient ssRNA delivery systems.
单链核糖核酸（ssRNA）可作为探针、反义核酸（AS）、微小RNA（miRNA）类似物及抑制剂，在基因治疗和分子诊断方面颇具前景。然而，游离的ssRNA由于带负电荷且酶稳定性差，导致细胞摄取能力不佳，这在很大程度上限制了ssRNA在生物医学中的实际应用。为解决这些问题，我们开发了一种聚乙二醇化还原氧化石墨烯（PEG-RGO）纳米载体，用于高效递送ssRNA。我们已证明，与广泛研究的聚乙二醇化氧化石墨烯（PEG-GO）相比，PEG-RGO具有卓越的ssRNA负载和递送能力。计算模拟进一步表明，PEG-RGO与ssRNA的结合比PEG-GO更强，这与实验结果一致。这些结果将为设计基于RGO的生物相容性高效ssRNA递送系统提供参考。
