功能性氧化石墨烯递送的基于质粒的Stat3小干扰RNA抑制小鼠恶性黑色素瘤细胞生长。
Plasmid-Based Stat3 siRNA Delivered by Functional Graphene Oxide Suppresses Mouse Malignant Melanoma Cell Growth.
作者信息
Yin Di, Li Yang, Guo Baofeng, Liu Zhewen, Xu Yang, Wang Xiaoqin, Du Yanwei, Xu Libo, Meng Yan, Zhao Xuejian, Zhang Ling
机构信息
Prostate Diseases Prevention and Treatment Research Centre and Department of Pathophysiology, Norman Bethune Medical School, Jilin University, Changchun, China.
出版信息
Oncol Res. 2016;23(5):229-36. doi: 10.3727/096504016X14550280421449.
RNA interference (RNAi) has been used for cancer gene therapy in recent years. However, the application of RNAi is hindered in the absence of safe and efficient gene delivery. In this article, a novel vehicle of graphene oxide functionalized with polyethylenimine and polyethylene glycol (GO-PEI-PEG) was successfully synthetized and then used to deliver plasmid-based Stat3 siRNA. The carrier can readily bind plasmid with high transfection efficiency. Moreover, molecular biology studies reveal that Stat3-related gene and protein expressions were significantly inhibited, suggesting that the formation of GO-PEI-PEG complexes could be utilized as a promising gene delivery in cancer therapy.
近年来，RNA干扰（RNAi）已被用于癌症基因治疗。然而，在缺乏安全有效的基因递送的情况下，RNAi的应用受到阻碍。在本文中，一种用聚乙烯亚胺和聚乙二醇功能化的新型氧化石墨烯载体（GO-PEI-PEG）被成功合成，然后用于递送基于质粒的Stat3小干扰RNA（siRNA）。该载体能够轻松地与质粒结合，具有高转染效率。此外，分子生物学研究表明，Stat3相关基因和蛋白表达受到显著抑制，这表明GO-PEI-PEG复合物的形成可作为一种有前景的癌症治疗基因递送方法。
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