Stewart Charles R
Department of BioSciences, Rice University, 6100 Main St., Houston, TX, 77251, USA.
Methods Mol Biol. 2018;1681:31-39. doi: 10.1007/978-1-4939-7343-9_2.
When Bacillus subtilis is infected by bacteriophage SPO1, the phage directs the remodeling of the host cell, converting it into a factory for phage reproduction. Much synthesis of host DNA, RNA, and protein is shut off, and cell division is prevented. Here I describe the protocols by which we have demonstrated those processes, and identified the roles played by specific SPO1 gene products in causing those processes.
当枯草芽孢杆菌被噬菌体SPO1感染时，该噬菌体会引导宿主细胞进行重塑，将其转变为一个噬菌体繁殖工厂。宿主细胞的DNA、RNA和蛋白质的大量合成被关闭，细胞分裂也被阻止。在此，我描述了我们用以证明这些过程，并确定特定SPO1基因产物在引发这些过程中所起作用的实验方案。
