Kostas Emily T, Wilkinson Stuart J, White Daniel A, Cook David J
International Centre for Brewing Science, Division of Food Science, The University of Nottingham, Sutton Bonington Campus, Loughborough, Leicestershire, LE12 5RD, UK.
Plymouth Marine Laboratory, Prospect Pl, Plymouth, Devon PL1 3DH, UK.
Methods Mol Biol. 2020;1980:181-190. doi: 10.1007/7651_2017_105.
Accurate quantification of the carbohydrate content of biomass is crucial for many bio-refining processes. The most commonly followed protocol is typically a modification of the NREL-based assay (specifically designed for carbohydrate analysis from lignocellulosic biomass). However, this NREL protocol was revealed to be excessively thermochemically harsh for seaweed biomass. This can result in erroneously low total sugar quantification as the reaction severity can degrade a proportion of the liberated sugars to decomposition products such as furans. Here we describe an optimization of the total acid hydrolysis protocol for accurate quantification of the carbohydrate content of seaweeds. Different species of seaweed can be accurately evaluated for their carbohydrate contents by following this optimized method.
准确量化生物质中的碳水化合物含量对于许多生物精炼过程至关重要。最常用的方案通常是对基于美国国家可再生能源实验室（NREL）的分析方法进行修改（该方法专门设计用于分析木质纤维素生物质中的碳水化合物）。然而，事实证明，这种NREL方案对海藻生物质的热化学作用过于强烈。这可能导致总糖定量错误地偏低，因为反应强度会使一部分释放出的糖降解为呋喃等分解产物。在此，我们描述了一种总酸水解方案的优化方法，用于准确量化海藻中的碳水化合物含量。通过遵循这种优化方法，可以准确评估不同种类海藻的碳水化合物含量。
