Van Wychen S, Laurens L M L
National Renewable Energy Laboratory, National Bioenergy Center, Golden, CO, USA.
Methods Mol Biol. 2020;1980:191-202. doi: 10.1007/7651_2017_106.
The carbohydrate fraction of microalgal biomass is complex and consists of a combination of neutral, acidic, amino sugars, and sugar alcohols. These carbohydrate amalgamations can be difficult to accurately quantify with the most analytical carbohydrate determination techniques. The method described here provides a straightforward two-step sulfuric acid hydrolysis followed by soluble carbohydrate quantification by either a spectrophotometric method (based on aldehyde functional group derivatization), for an overall combined monomeric sugar concentration determination, or a liquid chromatography method, for a more specific monomeric sugar profile.
微藻生物质的碳水化合物部分很复杂，由中性糖、酸性糖、氨基糖和糖醇组合而成。使用大多数分析碳水化合物的测定技术，这些碳水化合物的混合物可能难以准确量化。这里描述的方法提供了一个简单的两步硫酸水解法，随后通过分光光度法（基于醛官能团衍生化）进行可溶性碳水化合物定量，以测定总的单体糖浓度，或者通过液相色谱法，以获得更具体的单体糖谱。
