Beijing Key Laboratory of Farmland Soil Pollution Prevention and Remediation, College of Resources and Environmental Science, China Agricultural University, Beijing, P. R. China.
J Sep Sci. 2018 Feb;41(3):713-722. doi: 10.1002/jssc.201700798. Epub 2017 Dec 11.
A modified quick, easy, cheap, effective, rugged, and safe method was established for simultaneous extraction and cleanup of multiple antibiotics in leafy vegetables, and ultra-high performance liquid chromatography with tandem mass spectrometry was used for analysis. Antibiotics in leafy vegetables were extracted with citric acid/sodium citrate in mixed solvents consisting of acetonitrile/methanol (85:15, v/v) from 10 g of vegetables. Octadecylsilyl and graphitized carbon black were used as dispersant adsorbents. This method was able to effectively extract all of the target antibiotics from leafy vegetables. The average recoveries of 20 antibiotics ranged from 57 to 91%. The limits of detection were 0.33-2.92 μg/kg. The developed method subsequently demonstrated its selectivity, sensitivity, and reliability for detecting multiple antibiotics in 15 samples. Antibiotic residues in vegetables have attracted great concern with respect to human health. It is recommended that standards should be established for antibiotic residues in vegetables to ensure food safety.
建立了一种改良的快速、简便、价廉、有效、耐用和安全的方法，用于同时提取和净化叶菜类蔬菜中的多种抗生素，并采用超高效液相色谱-串联质谱法进行分析。从 10 克蔬菜中，用柠檬酸/柠檬酸钠在由乙腈/甲醇（85:15，v/v）组成的混合溶剂中提取叶菜类蔬菜中的抗生素。十八烷基硅烷和石墨化炭黑用作分散吸附剂。该方法能够有效地从叶菜类蔬菜中提取所有目标抗生素。20 种抗生素的平均回收率为 57%至 91%。检测限为 0.33-2.92μg/kg。该方法随后在 15 个样本中表现出了对多种抗生素检测的选择性、灵敏度和可靠性。蔬菜中的抗生素残留问题引起了人们对人类健康的极大关注。建议制定蔬菜中抗生素残留的标准，以确保食品安全。
