Owoeye Olatunde, Gabriel Micheal Oluwatosin
Department of Anatomy, College of Medicine, University of Ibadan, Ibadan.
Niger J Physiol Sci. 2017 Jun 30;32(1):97-104.
Ischaemic brain injury continues to be devastating, causing social, medical and relationship disruption. Oxidativedamage has been reported to be one of the mechanisms for brain damage following ischaemic stroke. The antioxidant activityof Hibiscus sabdariffa L. was investigated for a possible protective effect against ischaemia-induced brain damage in rats.Adult male Wistar rats (n=35) were divided into five groups of 7 rats per group. Group 1 served as control was given tapwater; Group 2: 500 mg/kg daily of Hibiscus sabdariffa L. extract (HSE); Group 3: bilateral common carotid artery occlusion(BCCAO) for 30 minutes followed by reperfusion for 24 hours; Group 4: 500 mg/kg (HSE) before BCCAO; Group 5: 500mg/kg vitamin E before BCCAO. All administrations were oral and lasted 3 weeks. Behavioural studies namely: transitions,rearings, groomings and forelimb grip strength were carried out. Rats were thereafter euthanized and biochemical[malondialdehyde (MDA), reduced glutathione (GSH), superoxide dismutase (SOD) and catalase (CAT)], histological andmorphological investigations were carried out on rat whole brain. Animals pretreated with HSE showed a significant (p<0.05)reduction in their body weight compared to the control group. BCCAO produced a significant (p<0.05) reduction in GSH,SOD and CAT while elevating MDA non-significantly. The HSE and Vitamin E pretreatment ameliorated these biochemicalalterations and also attenuated reactive changes in cortical neurons. BCCAO treatment increased grooming and forelimbstrength which both HSE and vitamin E pretreatment reversed. The results suggest that H. sabdariffa L and vitamin E were protective in acute cerebral ischaemia induced by bilateral common carotid artery occlusion in adult male rats.
缺血性脑损伤仍然具有毁灭性，会导致社会、医疗及人际关系的破坏。据报道，氧化损伤是缺血性中风后脑损伤的机制之一。研究了玫瑰茄的抗氧化活性，以探讨其对大鼠缺血性脑损伤的潜在保护作用。成年雄性Wistar大鼠（n = 35）分为五组，每组7只。第1组作为对照组，给予自来水；第2组：每日给予500 mg/kg玫瑰茄提取物（HSE）；第3组：双侧颈总动脉闭塞（BCCAO）30分钟，随后再灌注24小时；第4组：在BCCAO前给予500 mg/kg（HSE）；第5组：在BCCAO前给予500 mg/kg维生素E。所有给药均为口服，持续3周。进行了行为学研究，即：转换、竖毛、梳理和前肢握力测试。此后对大鼠实施安乐死，并对大鼠全脑进行生化（丙二醛（MDA）、还原型谷胱甘肽（GSH）、超氧化物歧化酶（SOD）和过氧化氢酶（CAT））、组织学和形态学研究。与对照组相比，用HSE预处理的动物体重显著降低（p<0.05）。BCCAO导致GSH、SOD和CAT显著降低（p<0.05），同时MDA略有升高。HSE和维生素E预处理改善了这些生化改变，也减轻了皮质神经元的反应性变化。BCCAO处理增加了梳理行为和前肢力量，而HSE和维生素E预处理均使其逆转。结果表明，玫瑰茄和维生素E对成年雄性大鼠双侧颈总动脉闭塞诱导的急性脑缺血具有保护作用。
