Department of chemistry, College of humanities and sciences, Nihon University, Tokyo, Japan.
Chem Rec. 2018 Apr;18(4):433-444. doi: 10.1002/tcr.201700046. Epub 2017 Nov 14.
Artificial and natural lipid membranes that elicit transmembrane signaling is are useful as a platform for channel-based biosensing. In this account we summarize our research on the design of transmembrane signaling associated with lipid bilayer membranes containing nanopore-forming compounds. Channel-forming compounds, such as receptor ion-channels, channel-forming peptides and synthetic channels, are embedded in planar and spherical bilayer lipid membranes to develop highly sensitive and selective biosensing methods for a variety of analytes. The membrane-bound receptor approach is useful for introducing receptor sites on both planar and spherical bilayer lipid membranes. Natural receptors in biomembranes are also used for designing of biosensing methods.
人工和天然的脂质膜可以引发跨膜信号转导，因此可用作基于通道的生物传感的平台。在本报告中，我们总结了我们在设计与包含形成纳米孔的化合物的双层脂质膜相关的跨膜信号转导方面的研究。将通道形成化合物（例如受体离子通道、通道形成肽和合成通道）嵌入到平面和球形双层脂质膜中，以开发针对各种分析物的高灵敏度和选择性生物传感方法。膜结合受体方法可用于在平面和球形双层脂质膜上引入受体位点。生物膜中的天然受体也可用于设计生物传感方法。
