Nishio Masato, Shoji Atsushi, Sugawara Masao
Department of Chemistry, College of Humanities and Sciences, Nihon University, Tokyo, Japan.
Anal Sci. 2012;28(7):661-7. doi: 10.2116/analsci.28.661.
The channel activity of gramicidin A in free-standing planar lipid bilayers with different charges of polar head groups and various lengths of hydrocarbon tails were analyzed in terms of the channel conductance, the lifetime of channel events and the magnitude of integrated currents. The channel activity of gramicidin A in lipid bilayers is tunable by adjusting the membrane composition. The in situ coupling of the anti-BSA antibody as a model protein to the amine moiety of phosphatidylethanolamine (PE) in a lipid bilayer by the amine coupling method allowed us to design an antigen (BSA)-sensitive interface, in which the integrated current, rather than the frequency of channel event, can be used as an analytical signal. The potential of the present system for highly sensitive and selective detection of BSA at 10(-9) g/mL level is demonstrated.
就通道电导、通道事件寿命和积分电流大小而言，分析了短杆菌肽A在具有不同极性头基团电荷和不同烃链长度的独立平面脂质双分子层中的通道活性。通过调节膜组成可调控短杆菌肽A在脂质双分子层中的通道活性。通过胺偶联法将作为模型蛋白的抗牛血清白蛋白（BSA）抗体原位偶联至脂质双分子层中磷脂酰乙醇胺（PE）的胺部分，这使我们能够设计出一种对抗原（BSA）敏感的界面，其中积分电流而非通道事件频率可作为分析信号。证明了本系统在10⁻⁹ g/mL水平对BSA进行高灵敏度和高选择性检测的潜力。
