Desai Karishma M, Angadi Punnya V, Kale Alka D, Hallikerimath Seema
Department of Oral Pathology and Microbiology, KLE University's KLE VK Institute of Dental Sciences and Hospital, Belagavi, India.
Acta Cytol. 2018;62(1):39-43. doi: 10.1159/000484195. Epub 2017 Nov 15.
Cell block preparation is a recognized technique in histopathological diagnosis. This technique aids in maintaining an intact architecture and reducing the diagnostic errors associated with fine-needle aspiration cytology. Numerous techniques have been put forth, but the need for an optimal technique for routine use in the laboratory still persists. The aim of our study was to establish a cell block technique which aids in the accurate diagnosis of head and neck pathology.
A modified cell block technique was developed using alcohol-formalin as a fixative. Forty fine-needle aspiration fluids from clinically and radiologically diagnosed cases of head and neck pathology were used as samples. The cell block sections were compared with the cytology smears to determine the utility of the technique.
The cell blocks presented with better preservation of the architectural framework and enabled a quick diagnosis. Cellular clumping was negligible, and nuclear as well as cellular details were maintained similar to tissue sections. It led to the integration of conventional techniques using 10% neutral buffered formalin with that of the 10% alcohol-formalin technique.
Modified cell block technique can be used as a simple and effective tool in the routine diagnosis of head and neck pathology.
细胞块制备是组织病理学诊断中一种公认的技术。该技术有助于保持完整的结构，并减少与细针穿刺细胞学相关的诊断错误。已经提出了许多技术，但实验室常规使用的最佳技术需求仍然存在。我们研究的目的是建立一种有助于准确诊断头颈部病变的细胞块技术。
使用酒精 - 福尔马林作为固定剂开发了一种改良的细胞块技术。将来自临床和放射学诊断的头颈部病变病例的40份细针穿刺液用作样本。将细胞块切片与细胞学涂片进行比较，以确定该技术的实用性。
细胞块呈现出更好的结构框架保存，并能够快速诊断。细胞聚集可忽略不计，细胞核和细胞细节与组织切片相似得以保留。它导致了使用10%中性缓冲福尔马林的传统技术与10%酒精 - 福尔马林技术的整合。
改良的细胞块技术可作为头颈部病变常规诊断中的一种简单有效的工具。
