Department of Oral Pathology and Microbiology, Bharati Vidyapeeth (Deemed to Be University) Dental College and Hospital, Sangli, India.
Department of Oral Pathology and Microbiology, Bharati Vidyapeeth (Deemed to Be University) Dental College and Hospital, Pune, India.
Biotech Histochem. 2021 Apr;96(3):197-201. doi: 10.1080/10520295.2020.1780314. Epub 2020 Jun 18.
Cell blocks and fine needle aspirations can be used for cytopathological diagnosis. Conventional fine needle aspiration smears provide limited material for diagnosis. The cell block technique provides more tissue, which improves diagnostic accuracy. We compared a modified cell block cytology to fine needle aspiration for providing optimal preservation of histochemical and immunocytochemical properties. We used 30 fine needle aspirates from oral lesions in two groups: group 1, fine needle aspiration cytology; group 2, cell block cytology. Smears of fine needle aspirates were stained with Papanicolaou. For the modified cell block technique, aspirated material was centrifuged to create a cell pellet, which then was fixed with Nathan alcohol formalin substitute. After routine histopathological processing, cell pellets were embedded in paraffin, then sectioned and stained with hematoxylin and eosin. Sections were compared to Papanicolaou stained smears of fine needle samples. Cellular morphology and staining quality of modified cell block samples were superior to fine needle aspiration cytology; both methods exhibited distinct nuclear morphology. Modified cell blocks provide excellent cytopathologic features compared to fine needle aspiration cytology.
细胞块和细针吸取物可用于细胞病理学诊断。传统的细针吸取涂片为诊断提供的材料有限。细胞块技术可提供更多的组织，从而提高诊断准确性。我们比较了改良的细胞块细胞学与细针抽吸术，以获得最佳的组织化学和免疫细胞化学特性保存。我们使用两组 30 例口腔病变的细针抽吸物：第 1 组，细针抽吸细胞学；第 2 组，细胞块细胞学。细针抽吸物的涂片用巴氏染色。对于改良的细胞块技术，将抽吸的材料离心以形成细胞沉淀，然后用 Nathan 酒精福尔马林替代物固定。常规组织病理学处理后，将细胞沉淀包埋在石蜡中，然后切片并用苏木精和伊红染色。将切片与细针样本的巴氏染色涂片进行比较。改良细胞块样本的细胞形态和染色质量优于细针抽吸细胞学；两种方法均显示出明显的核形态。与细针抽吸细胞学相比，改良细胞块可提供出色的细胞病理学特征。
