红色荧光发射的 BSA 稳定的铜纳米团簇可作为一种灵敏的探针，用于芦丁的荧光传感和可视化成像检测。
Red-emitting BSA-stabilized copper nanoclusters acted as a sensitive probe for fluorescence sensing and visual imaging detection of rutin.
机构信息
College of Chemistry and Chemical Engineering, Shandong Sino-Japanese Center for Collaborative Research of Carbon Nanomaterials, Laboratory of Fiber Materials and Modern Textile, The Growing Base for State Key Laboratory, Qingdao University, Shandong 266071, PR China.
College of Chemistry and Chemical Engineering, Shandong Sino-Japanese Center for Collaborative Research of Carbon Nanomaterials, Laboratory of Fiber Materials and Modern Textile, The Growing Base for State Key Laboratory, Qingdao University, Shandong 266071, PR China.
出版信息
Talanta. 2018 Feb 1;178:1006-1010. doi: 10.1016/j.talanta.2017.08.102. Epub 2017 Sep 1.
In this article, red-emitting copper nanoclusters (Cu NCs) were prepared in the matrix of bovine serum albumin (BSA) through using NH·HO as a reducing agent and BSA as a stabilizer. In the aqueous suspension of BSA-Cu NCs, the addition of rutin enabled the combination of rutin with BSA around Cu NCs via hydrogen bond and electrostatic interactions between functional groups of BSA and rutin. The approaching of rutin to Cu NCs induced that the fluorescence (FL) of Cu NCs was quenched by rutin due to the electron transfer from Cu NCs to rutin. Under the optimal conditions, the FL of Cu NCs was linearly decreased with the increase of rutin concentration from 0.1 to100 μM, together with a low detection limit of ca. 0.02μM. The FL quenching responses of Cu NCs were highly selective and sensitive towards rutin, over potential interferents in real human serum samples. Based on BSA-Cu NCs, this as-proposed FL probe exhibited a superior capability of rutin detection in real human serum samples. Significantly, this probe of rutin could realize the FL sensing of rutin and the naked-eye visual FL imaging detection of rutin in aqueous solution and on filter paper.
在本文中，通过使用 NH·HO 作为还原剂和 BSA 作为稳定剂，在牛血清白蛋白 (BSA) 的基质中制备了红色发射铜纳米团簇 (Cu NCs)。在 BSA-Cu NCs 的水悬浮液中，芦丁的加入使得芦丁通过 BSA 和 Cu NCs 之间的氢键和 BSA 与芦丁之间的官能团的静电相互作用与 BSA 结合。芦丁接近 Cu NCs 导致 Cu NCs 的荧光 (FL) 被芦丁猝灭，这是由于电子从 Cu NCs 转移到芦丁。在最佳条件下，Cu NCs 的 FL 随着芦丁浓度从 0.1 到 100 μM 的增加而线性降低，检测限约为 0.02 μM。Cu NCs 的 FL 猝灭响应对芦丁具有高度选择性和灵敏度，对实际人血清样品中的潜在干扰物也是如此。基于 BSA-Cu NCs，这种拟议的 FL 探针在实际人血清样品中表现出优异的芦丁检测能力。值得注意的是，该芦丁探针可实现芦丁的 FL 传感和芦丁在水溶液和滤纸上的裸眼可视化 FL 成像检测。
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