基于 BSA 稳定的铜纳米簇的棉酚高灵敏和选择性荧光检测。

Highly Sensitive and Selective Fluorescent Detection of Gossypol Based on BSA-Stabilized Copper Nanoclusters.

机构信息

State Key Laboratory of Cotton Biology, Institute of Cotton Research of CAAS, Anyang 455000, China.

Research Base, State Key Laboratory of Cotton Biology, Zhengzhou University, Zhengzhou 450001, Henan, China.

出版信息

Molecules. 2018 Dec 28;24(1):95. doi: 10.3390/molecules24010095.

DOI:10.3390/molecules24010095

PMID:30597835

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6337446/

Abstract

In this paper, fluorescent copper nanoclusters (NCs) are used as a novel probe for the sensitive detection of gossypol for the first time. Based on a fluorescence quenching mechanism induced by interactions between bovine serum albumin (BSA) and gossypol, fluorescent BSA-Cu NCs were seen to exhibit a high sensitivity to gossypol in the range of 0.1⁻100 µM. The detection limit for gossypol is 25 nM at a signal-to-noise ratio of three, which is approximately 35 times lower than the acceptable limit (0.9 µM) defined by the US Food and Drug Administration for cottonseed products. Moreover, the proposed method for gossypol displays excellent selectivity over many common interfering species. We also demonstrate the application of the present method to the measurement of several real samples with satisfactory recoveries, and the results agree well with those obtained using the high-performance liquid chromatography (HPLC) method. The method based on Cu NCs offers the followings advantages: simplicity of design, facile preparation of nanomaterials, and low experimental cost.

摘要

本文首次将荧光铜纳米簇（NCs）用作棉酚灵敏检测的新型探针。基于牛血清白蛋白（BSA）与棉酚相互作用诱导的荧光猝灭机制，荧光 BSA-Cu NCs 对 0.1⁻100 µM 范围内的棉酚表现出高灵敏度。棉酚的检测限为 25 nM（信噪比为 3），约比美国食品和药物管理局规定的棉籽产品可接受限值（0.9 µM）低 35 倍。此外，该方法对许多常见干扰物质具有优异的选择性。我们还将本方法应用于几种实际样品的测量，得到了令人满意的回收率，结果与高效液相色谱（HPLC）法吻合较好。基于 Cu NCs 的方法具有设计简单、纳米材料易于制备和实验成本低等优点。