Yuan Jingxue, Feng Yao, Hu Qingqing, Kuang Jianhua, Cheng Zhengjun
Chemical Synthesis and Pollution Control Key Laboratory of Sichuan Province, China West Normal University, Nanchong, 637002, China.
Institute of Applied Chemistry, China West Normal University, Nanchong, 637002, China.
J Fluoresc. 2025 Feb;35(2):987-995. doi: 10.1007/s10895-024-03582-3. Epub 2024 Jan 17.
In this study, a novel double-emission fluorescence probe at 340 and 400 nm was synthesized by one-pot method using phenylalanine (Phe) and ascorbic acid (AA) as stabilizing and reducing agents. It was found that the fluorescence intensity of the probe at 400 nm could be controlled by controlling the temperature within a certain range, and the ratio of double-emission fluorescence probe could be further regulated. Under the optimal conditions, the fluorescence intensity at 340 nm decreased significantly, while it only showed a slight decrease at 400 nm, which constituted the ratio fluorescence probe. The synthesized fluorescence probe showed good linearity in the range of 0.2-32 μM, and its detection limit was 63.4 nM. Moreover, the method was successfully employed to determine VA in vanilla drink and perfumes, and corresponding results were consistent with those of HPLC.
在本研究中，以苯丙氨酸（Phe）和抗坏血酸（AA）作为稳定剂和还原剂，采用一锅法合成了一种新型的在340和400 nm处有双发射的荧光探针。研究发现，在一定温度范围内，通过控制温度可以调控该探针在400 nm处的荧光强度，进而进一步调节双发射荧光探针的比例。在最佳条件下，340 nm处的荧光强度显著降低，而400 nm处仅略有下降，构成了比率荧光探针。合成的荧光探针在0.2 - 32 μM范围内呈现良好的线性，其检测限为63.4 nM。此外，该方法成功用于测定香草饮料和香水中的维生素A，相应结果与高效液相色谱法的结果一致。
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