一种用于检测细胞内半胱氨酸和高半胱氨酸的线粒体靶向比率型双光子荧光探针。
A mitochondria-targeted ratiometric two-photon fluorescent probe for detecting intracellular cysteine and homocysteine.
机构信息
School of Chemistry and Chemical Engineering & Center for Atomic Engineering of Advanced Materials & AnHui Province Key Laboratory of Chemistry for Inorganic/Organic Hybrid Functionalized Materials, Anhui University, Hefei 230601, PR China.
Jiangsu Collaborative Innovation Center for Ecological Building Materials and Environmental Protection Equipments, Yancheng Institute of Technology, Yancheng 224051, PR China.
出版信息
Talanta. 2018 Feb 1;178:24-30. doi: 10.1016/j.talanta.2017.08.085. Epub 2017 Aug 30.
A novel mitochondria-targeted ratiometric two-photon fluorescent probe (Mito-MQ) for detecting intracellular cysteine (Cys) and homocysteine (Hcy) has been designed. Mito-MQ showed the ratiometric fluorescent detection signal (the green-to-blue emissionfrom 517nm to 460nm) to cysteine (Cys) and homocysteine (Hcy) over glutathione (GSH), along with the fast response rate (10min). The detection mechanism was illustrated by H NMR, ESI-MS and theoretical calculation. The co-localization coefficient of 0.87 between Mito-MQ and MitoTracker Red revealed that the probe was predominantly present in mitochondria, therefore, Mito-MQ was successfully applied to detect mitochondrial oxidative stress by detecting the change of Cys/Hcy. Moreover, imaging in fresh tissue slices indicated that Mito-MQ could work in deep tissue (ca. 130µm) under two-photon excitation. Furthermore, the measurement of Cys/Hcy detection in zebrafish showed that probe can be used in determination of biothiols in vivo.
设计了一种新型的线粒体靶向比率型双光子荧光探针（Mito-MQ），用于检测细胞内半胱氨酸（Cys）和同型半胱氨酸（Hcy）。Mito-MQ 对半胱氨酸（Cys）和同型半胱氨酸（Hcy）具有比率型荧光检测信号（从 517nm 到 460nm 的绿色到蓝色发射），对谷胱甘肽（GSH）具有快速响应速度（10min）。通过 1 H NMR、ESI-MS 和理论计算说明了检测机制。Mito-MQ 与 MitoTracker Red 的共定位系数为 0.87，表明探针主要存在于线粒体中，因此，Mito-MQ 成功地通过检测 Cys/Hcy 的变化来检测线粒体氧化应激。此外，在新鲜组织切片中的成像表明，Mito-MQ 可以在双光子激发下在深部组织（约 130µm）中工作。此外，在斑马鱼中的 Cys/Hcy 检测测量表明，探针可用于体内生物硫醇的测定。
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